58365

SUPELCOSIL^™ LC-Si (5 µm) HPLC Columns

L × I.D. 25 cm × 10 mm, HPLC Column

• UNSPSC Code: 41115700
• eCl@ss: 32110501

Properties

• product name: SUPELCOSIL^™ LC-Si HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm
• Agency: suitable for USP L3
• Quality Level: 100
• manufacturer/tradename: SUPELCOSIL^™
• parameter: ≤70 °C temp. limit; 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable
• L × I.D.: 25 cm × 10 mm
• surface area: 170 m²/g
• matrix: silica gel, spherical particle platform
• matrix active group: silica phase
• particle size: 5 μm
• pore size: 120 Å pore size
• pH range: 2-7.5
• application(s): food and beverages
• separation technique: hydrophilic interaction (HILIC); normal phase

Description

General description

A typical mobile phase used with normal-phase silica columns consists of a hydrocarbon solvent such as hexane or heptane, mixed with a relatively small percentage of more polar solvent. Non-polar compounds will elute from the column first, while polar solutes show stronger interaction with the silanol groups on the silica surface. The polar selectivity of silica is very helpful for separating mycotoxins. Silica is frequently used in preparative chromatography due to its low operating cost and back pressure compared to reversed-phase columns. Silica is particularly popular among organic chemists as a low-pressure preparative tool. The unmodified silica in SUPELCOSIL LC-Si columns also separate positional isomers.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC