581324-U

Ascentis^® C18 (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

• Synonym(s): Ascentis RP18 HPLC Column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: Ascentis^® C18 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L1 (Similar to Phenomenex Luna C18)
• product line: Ascentis^®
• feature: endcapped
• manufacturer/tradename: Ascentis^®
• packaging: 1 ea of
• extent of labeling: 25% Carbon loading
• parameter: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable; LC/MS: suitable
• L × I.D.: 15 cm × 4.6 mm
• surface area: 450 m²/g
• surface coverage: 3.7 μmol/m²
• impurities: <5 ppm metals
• matrix: fully porous particle; silica gel high purity, spherical
• matrix active group: C18 (octadecyl) phase
• particle size: 5 μm
• pore size: 100 Å
• operating pH range: 2-8
• application(s): food and beverages
• separation technique: reversed phase

Description

General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.

Application

• Preparation of polar embedded C18 stationary phase for efficient separation of peptides and proteins in high performance liquid chromatography.: This study details the development of a polar embedded C18 stationary phase, demonstrating its effectiveness in separating peptides and proteins. The Ascentis^® C18 HPLC Column was utilized for its high separation efficiency, crucial for proteomics and biopharmaceutical applications (Ali et al., 2022).


• A dilute-and-shoot liquid chromatography-tandem mass spectrometry method for urinary 18-hydroxycortisol quantification and its application in establishing reference intervals.: Researchers used the Ascentis^® C18 HPLC Column to develop a sensitive method for quantifying urinary 18-hydroxycortisol. This method aids in clinical diagnostics and endocrinological studies by providing accurate reference intervals (Zhao et al., 2022).


• A Comparative Study on Analysis of Ginsenosides in American Ginseng Root Residue by HPLC-DAD-ESI-MS and UPLC-HRMS-MS/MS.: This comparative analysis utilized the Ascentis^® C18 HPLC Column to separate and identify ginsenosides in American ginseng. The study highlights the column′s application in natural product research and quality control in the pharmaceutical industry (Hsu et al., 2022).


• Validation of a Rapid and Easy-to-Apply Method to Simultaneously Quantify Co-Loaded Dexamethasone and Melatonin PLGA Microspheres by HPLC-UV: Encapsulation Efficiency and In Vitro Release.: This research validates an HPLC method using the Ascentis^® C18 Column to simultaneously quantify dexamethasone and melatonin in PLGA microspheres, offering a robust tool for drug delivery studies (Brugnera et al., 2022).


• Simultaneous determination of ivermectin, clorsulon and their related substances in an injectable finished product by a stability-indicating RP-HPLC method.: The Ascentis^® C18 HPLC Column was employed to develop a stability-indicating method for analyzing ivermectin and clorsulon in pharmaceutical formulations, emphasizing its role in quality control and regulatory compliance (Padivitage et al., 2022).

Features and Benefits

• Excellent retention
• Symmetric peak shape
• High reproducibility
• Complete LC-MS compatibility

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable