Proteomics Dynamic Range Standard Set

Name: Proteomics Dynamic Range Standard Set
Brand: SIGMA

Protein Mass Spectrometry Calibration Standard

Synonym(s):

Dynamic Range Standards, Proteomics Standard Set

Select a Size

Change View

Pack Size	SKU	Availability	Price

About This Item

UNSPSC Code:

12161503

EC Number:

232-650-8

NACRES:

NA.24

Form:

ready-to-use solution

Biological source:

human

Pricing and availability is not currently available.

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

human

Quality Segment

200

form

ready-to-use solution

quality

Protein Mass Spectrometry Calibration Standard

concentration

10.6 μg/ampule protein

technique(s)

mass spectrometry (MS): suitable

shipped in

wet ice

storage temp.

−20°C

Description

General description

The Proteomics Dynamic Range Standard Set is produced from a mixture of 47 individual human source or human sequence recombinant proteins, each of which has been selected to limit heterogeneous post-translational modifications (PTMs). The protein standard is formulated from 6 mixtures of 8 proteins to present a dynamic range of 5 orders of magnitude, ranging from 50 pmoles to 500 amoles. Each protein has been quantitated by amino acid analysis (AAA) prior to formulation.

Application

Proteomics Dynamic Range Standard Set has been used in the intensity-based absolute quantification (iBAQ) of E .coli proteins,[1] embryonic stem cells (ESCs) and neuronal precursor cells (NPCs) proteomes. It has also been used as a standard to spike HeLa cells for label-free quantification.

The Proteomics Dynamic Range Standard Set can be used to standardize and/or evaluate mass spectrometric (e.g., LC-MS/MS, MALDI-TOF-MS, etc.) and electrophoretic analysis conditions prior to the analysis of complex protein samples. UPS2 can be used to bracket precious experimental data sets between runs of a known complex standard sample. This allows confirmation of the robustness of the analysis method and stability of the instrument employed. Additionally, laboratories generating or comparing mass spectrometric data derived from poorly defined samples can use UPS2 as an external reference to assist with the evaluation of results and experimental methodology.
Proteomics Dynamic Range Standard Set has been used for the quantification of dynamic range universal protein standard on Orbitrap Analyzer using all ion fragmentation.[2] It has been used as a standard for intensity-based absolute quantification of proteins (iBAQ) in LC-MS (liquid chromatography-mass spectrometry)/MS analysis.[1]

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	UPS1	EMS0004	EMS0005
Sigma-Aldrich UPS2 Proteomics Dynamic Range Standard Set	Sigma-Aldrich UPS1 Universal Proteomics Standard Set	Sigma-Aldrich EMS0004 SOLu-Trypsin	Sigma-Aldrich EMS0005 SOLu-Trypsin Dimethylated
technique(s) mass spectrometry (MS): suitable	technique(s) mass spectrometry (MS): suitable	technique(s) -	technique(s) -
biological source human	biological source -	biological source -	biological source -
concentration 10.6 μg/ampule protein	concentration -	concentration -	concentration -
form ready-to-use solution	form ready-to-use solution	form ready-to-use solution	form ready-to-use solution
shipped in wet ice	shipped in -	shipped in wet ice	shipped in wet ice
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C