SAE0214

Leucoagglutinin from P. vulgaris, recombinant

Expressed in P. pastoris, lyophilized powder, suitable for cell culture

• Synonym(s): PHA-L, Phytohemagglutinin-L, Red Kidney Bean Lectin
• UNSPSC Code: 12352202
• NACRES: NA.21

Properties

• Quality Level: 200
• form: lyophilized powder
• UniProt accession no.: P05087
• shipped in: wet ice
• storage temp.: -10 to -25°C

Description

General description

Lectin from Red Kidney Bean has long been used in a wide variety of applications that require carbohydrate binding including but not limited to T-cell proliferation, leucoagglutination, glycoconjugate capture and more. The native form of lectin from Red Kidney Bean is composed of 2 isolectins (PHA-L and PHA-E) which combine in heterotetromeric combinations that elicit a variety of properties. This recombinant form is strictly the leucoagglutinin (PHA-L) isolectin only, so the erythroagglutinin (PHA-E) isolectin is absent in this product. This allows for more selective performance because there is no chance of PHA-E contamination as seen in native source products.

Biochem/physiol Actions

Kidney bean lectin has a history of being used as a phytohemagglutinin because of its ability to agglutinate blood cells. This isolectin (PHA-L) specficially is the Leucoagglutinin type and can be used for leucoagglutination and as a mitogen. As a lectin, it is known to have some selectivity for binding of β1-6 branched N-glycans.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable