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Q1754

Sigma-Aldrich

Q Sepharose High Performance

preswollen, 24-44 μm (wet), average exclusion limit ~4,000,000 Da

Synonym(s):

Q Sepharose

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About This Item

MDL number:
UNSPSC Code:
47101511
NACRES:
NA.56

form

preswollen

Quality Level

technique(s)

RNA extraction: suitable

matrix active group

, —CH2N+(CH3)3

particle size

24-44 μm (wet)

pore size

~4,000,000 Da average exclusion limit

pH

2—12

capacity

150-200 μeq/mL, gel

storage temp.

2-8°C

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Application

Q Sepharose is used in protein chromatography, ion exchange chromatography and anion exchange media. Q Sepharose has been used to study compounds of plant defense with applications for natural control of phytopathogenic fungi. Q Sepharose has also been used to develop an efficient method for extracting high-quality mRNA from soil and to study the immunomodulatory proteins from garlic (Allium sativum).

Physical form

Preswollen in 20% ethanol

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

100.4 - 109.4 °F

Flash Point(C)

38 - 43 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Fatima Clement et al.
International immunopharmacology, 10(3), 316-324 (2009-12-17)
Garlic (Allium sativum), an important medicinal spice, displays a plethora of biological effects including immunomodulation. Although some immunomodulatory proteins from garlic have been described, their identities are still unknown. The present study was envisaged to isolate immunomodulatory proteins from raw
Suzana M Ribeiro et al.
Peptides, 32(5), 868-874 (2010-10-20)
Antifungal proteins and peptides, essential compounds for plant defense, have been isolated from several tissues of various plants. These proteins could be used as a natural alternative to control phytopathogenic fungi. In this report a heterodimeric antifungal protein named Pa-AFP1
Carsten Mettel et al.
Applied and environmental microbiology, 76(17), 5995-6000 (2010-07-14)
Here, we report an efficient method for extracting high-quality mRNA from soil. Key steps in the isolation of total RNA were low-pH extraction (pH 5.0) and Q-Sepharose chromatography. The removal efficiency of humic acids was 94 to 98% for all

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