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M2393

Sigma-Aldrich

Magnesium chloride hexahydrate

BioReagent, suitable for cell culture, suitable for insect cell culture

Synonym(s):

MgCl2 hexahydrate, MgCl2.6H2O

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About This Item

Linear Formula:
MgCl2 · 6H2O
CAS Number:
Molecular Weight:
203.30
EC Number:
MDL number:
UNSPSC Code:
12352302
PubChem Substance ID:
NACRES:
NA.71
grade:
reagent
solubility:
H2O: soluble 500 mg/mL, clear, colorless
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grade

reagent

product line

BioReagent

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

solubility

H2O: soluble 500 mg/mL, clear, colorless

SMILES string

O.O.O.O.O.O.Cl[Mg]Cl

InChI

1S/2ClH.Mg.6H2O/h2*1H;;6*1H2/q;;+2;;;;;;/p-2

InChI key

DHRRIBDTHFBPNG-UHFFFAOYSA-L

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Application

Magnesium chloride hexahydrate (MgCl2·6H2O) has been used:
  • to prepare electrochemical substrate solution for surface treatment and immunoassay preparation of monoclonal anti-creatine kinase (CK-MB) antibody
  • as a component of casamino acids medium (Hv-Ca) to culture Haloferax volcanii strains
  • to prepare hydrated-salt poly(vinylidenefluoride)-hexafluoropropylene P(VDF-HFP)/MgCl2·6H2O nanocomposite film to study its phase transformation, surface morphology and dielectric property

Biochem/physiol Actions

Magnesium chloride is commonly used as a reagent in chemistry and molecular biology as a source of magnesium ions. Magnesium exhibits several biological roles in enzymology, cell membrane and wall structural integrity, muscle cell physiology, and nucleic acid structure. It functions as a co-factor in various enzymes, like deoxyribonuclease (DNAse), the restriction enzymes EcoRI and EcoRV, and Ribonuclease H. Magnesium stabilizes polymeric nucleic acids including ribozymes and transfer RNA.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Vladimir Riazanski et al.
iScience, 23(11), 101759-101759 (2020-12-01)
The relative contribution of the two phagosomal catabolic processes, oxidative and metabolic, was assessed in the killing of Pseudomonas aeruginosa in phagosomes of alveolar macrophages (AMs) from wild-type (p47-phox +/+ ) or NOX-defective (p47-phox -/- ) mice. Free radical release
Roshali T de Silva et al.
Microbiology (Reading, England), 167(2) (2021-01-19)
Some microbes display pleomorphism, showing variable cell shapes in a single culture, whereas others differentiate to adapt to changed environmental conditions. The pleomorphic archaeon Haloferax volcanii commonly forms discoid-shaped ('plate') cells in culture, but may also be present as rods
Alissa C Rothchild et al.
Bio-protocol, 10(10) (2020-10-01)
Mycobacterium tuberculosis (Mtb) is transmitted by aerosol and can cause serious bacterial infection in the lung that can be fatal if left untreated. Mtb is now the leading cause of death worldwide by an infectious agent. Characterizing the early events
Goli Ardestani et al.
Cell calcium, 87, 102181-102181 (2020-02-26)
Prior to maturation, mouse oocytes are arrested at the germinal vesicle (GV) stage during which they experience constitutive calcium (Ca2+) influx and spontaneous Ca2+ oscillations. The oscillations cease during maturation but Ca2+ influx continues, as the oocytes' internal stores attain
Huiwen Hao et al.
EMBO reports, 21(3), e48385-e48385 (2020-01-28)
Microtubules derived from the Golgi (Golgi MTs) have been implicated to play critical roles in persistent cell migration, but the underlying mechanisms remain elusive, partially due to the lack of direct observation of Golgi MT-dependent vesicular trafficking. Here, using super-resolution

Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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