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D9443

SAFC

Dulbecco′s Modified Eagle′s Medium - low glucose

liquid, sterile-filtered, suitable for cell culture, designed for isotope labeling in cell culture applications

Synonym(s):

DME, DMEM

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About This Item

MDL number:
MFCD00217342
UNSPSC Code:
12352207
NACRES:
NA.75

description

With 1000mg/L D-glucose, L-glutamine, and sodium bicarbonate. Without arginine, leucine, lysine, sodium pyruvate, and phenol red

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: 3.7 g/L
sodium pyruvate: no
glucose: 1.0 g/L
L-glutamine: 0.584 g/L
phenol red: no
HEPES: no

shipped in

ambient

storage temp.

2-8°C

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Reconstitution

Designed for use in stable isotope labeling with amino acids in cell culture applications.
Supplement with 0.100 g/L L-Arginine-13C6, 15N4 HCl (Product 608033), 0.100 g/L L-Leucine-13C6, 15N (Product 608068), and 0.100 g/L L-Lysine-13C6, 15N2 (Product 608041).

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Mahesh Saqcena et al.
PloS one, 8(8), e74157-e74157 (2013-08-27)
In multicellular organisms, cell division is regulated by growth factors (GFs). In the absence of GFs, cells exit the cell cycle at a site in G1 referred to as the restriction point (R) and enter a state of quiescence known
A M Edick et al.
Journal of dairy science, 104(1), 1123-1135 (2020-11-10)
The objective of this study was to determine the role of GCN2 in the response to AA deprivation of primary bovine mammary epithelial cells (BMEC). Cells were isolated from the mammary tissue of 2 lactating Holstein cows by enzymatic digestion
Dipsikha Biswas et al.
Cell death discovery, 7(1), 241-241 (2021-09-17)
Triple-negative breast cancers (TNBCs) are characterized by poor survival, prognosis, and gradual resistance to cytotoxic chemotherapeutics, like doxorubicin (DOX). The clinical utility of DOX is limited by its cardiotoxic and chemoresistant effects that manifest over time. To induce chemoresistance, TNBC
Sona Hubackova et al.
Molecular oncology, 13(7), 1467-1489 (2019-03-29)
Radiation and chemotherapy represent standard-of-care cancer treatments. However, most patients eventually experience tumour recurrence, treatment failure and metastatic dissemination with fatal consequences. To elucidate the molecular mechanisms of resistance to radio- and chemotherapy, we exposed human cancer cell lines (HeLa
Dipsikha Biswas et al.
The Journal of biological chemistry, 295(46), 15597-15621 (2020-09-04)
Branched-chain α-keto acids (BCKAs) are catabolites of branched-chain amino acids (BCAAs). Intracellular BCKAs are cleared by branched-chain ketoacid dehydrogenase (BCKDH), which is sensitive to inhibitory phosphorylation by BCKD kinase (BCKDK). Accumulation of BCKAs is an indicator of defective BCAA catabolism
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