AFRC MAC 77
85102262, Reactivity: Anti-Nitrate Reductase
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About This Item
UNSPSC Code:
41106514
Recommended Products
biological source
Rat x Rat Hybridoma
ImmunoDon
F344 rat spleen
Myeloma
Y3Ag1.2.3
Reactivity
Anti-Nitrate Reductase
technique(s)
cell culture | mammalian: suitable
isotype
IgG2b
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat Y3Ag1.2.3 x Rat F344 spleen
Cell Line Description
The antibody specific for NADH nitrate reductase and inhibits the reduction of nitrate to nitrite using NADH as electron donor. It also inhibits the reduction of nitrate using flavin nucleotides (FMNH2 or FADH2) and methyl viologen. The antibody is directed against NADH-NR of mono and dicotyledons.
Immunogen
Nitrate reductase purified from the leaves of 6 to 8 week old spinach plants - Spinacea oleracea l
Culture Medium
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
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Certificates of Analysis (COA)
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