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Key Documents

A6358

Sigma-Aldrich

Anti-Atg14 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-KIAA0831

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~60 kDa

species reactivity

mouse, human

technique(s)

western blot: 1:150-1:300 using whole extracts of mouse 3T3 cells
western blot: 1:250-1:500 using whole extracts of human HeLa cells.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Related Categories

General description

Autophagy-specific subunit of the PtdIns3K complex (Atg14) is encoded by the gene mapped to human chromosome 14q22.3. Atg14, also known as KIAA0831, is an autophagy protein.

Specificity

Anti-Atg14 recognizes human and mouse Atg14.

Application

Anti-Atg14 antibody produced in rabbit has been used in western blotting.

Biochem/physiol Actions

Autophagy Related 14 (Atg14) is an essential gene for autophagy. Atg14 is present on autophagic isolation membranes and is required for autophagosome formation. Atg14 together with vacuolar protein sorting-associated protein 34 (Vps34) and Atg6/Vps30 forms part of a phosphoinositide 3(PI3)-kinase complex in yeast. Two distinct PI3-kinase complexes, complex I and II, were identified. Complex I is involved in autophagy, whereas complex II is involved in vacuolar protein sorting. In mammals, homologs of Vps34, Vps15, Vps30/Atg6, Atg14 and Vps38 were identified as Vps34, p150, Beclin 1, Atg14 and UV radiation resistance associated gene (UVRAG), respectively. Although both Atg14 and UVRAG interact with Beclin 1, they are not present in the same complex. While Atg14 is present on autophagic isolation membranes, UVRAG mainly localized to Ras-related protein Rab9-positive endocytic compartments.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Angiotensin II suppresses autophagy and disrupts ultrastructural morphology and function of mitochondria in mouse skeletal muscle
KAS Silva, et al.
Journal of Applied Physiology, 126(6), 1550-1562 (2019)
Investigation of Androgen Responsive Elements in Some Autophagy Related Genes via In Silico Analysis.
Erzurumlu Y
S Int. Rev. Immunol., 11(1) (2020)
Atg14 and UVRAG: mutually exclusive subunits of mammalian Beclin 1-PI3K complexes
Itakura E and Mizushima N
Autophagy, 5(4), 534-536 (2009)
Hong-Min Ni et al.
The American journal of pathology, 183(6), 1815-1825 (2013-10-08)
Autophagy is a lysosomal degradation process that degrades long-lived cellular proteins and damaged organelles as a critical cell survival mechanism in response to stress. We recently reported that acute ethanol induces autophagy, which then reduces ethanol-induced liver injury. However, the
Atg14: a key player in orchestrating autophagy
Obara K and Ohsumi Y
International Journal of Cell Biology, 2011 (2011)

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