85707

2,5-Dihydroxybenzoic acid

matrix substance for MALDI-MS, >99.0% (HPLC)

• Synonym(s): 2,5-DHBA, DHB, Gentisic acid, Hydroquinonecarboxylic acid
• Linear Formula: (HO)₂C₆H₃CO₂H
• CAS Number: 490-79-9
• Molecular Weight: 154.12
• Beilstein: 2209119
• EC Number: 207-718-5
• MDL number: MFCD00002460
• UNSPSC Code: 12000000
• PubChem Substance ID: 329769406
• NACRES: NA.21

Properties

• grade: matrix substance for MALDI-MS
• Assay: >99.0% (HPLC)
• form: powder
• analyte functional class(es): polymers
• analyte chemical class(es): glycans, lipids, organic molecules, peptides, proteins
• technique(s): MALDI-MS: suitable
• mp: 200-207 °C; 204-208 °C (lit.)
• cation traces: Ba: ≤5 mg/kg; Ca: ≤5 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; K: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Na: ≤5 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Zn: ≤5 mg/kg
• ε (extinction coefficient): >2000 at 337 nm
• SMILES string: OC(=O)c1cc(O)ccc1O
• InChI: 1S/C7H6O4/c8-4-1-2-6(9)5(3-4)7(10)11/h1-3,8-9H,(H,10,11)
• InChI key: WXTMDXOMEHJXQO-UHFFFAOYSA-N

Description

Application

2,5-Dihydroxybenzoic acid is an ionic liquid matrix used for the analysis of biomolecules using matrix-assisted laser desorption/ionization combined with mass spectrometry.

Most commonly used matrix for carbohydrates. For MALDI-MS of free neutral glycans. Produces [M+Na]⁺ ion; may show a weaker [M+K]⁺ ion. Other species may be generated by the addition of the appropriate inorganic salt.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H302
• Precautionary Statements: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves