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MAB3876

Sigma-Aldrich

Anti-Myogenin Antibody

Chemicon®, from mouse

Synonym(s):

Anti-MYF4, Anti-bHLHc3, Anti-myf-4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

monoclonal

species reactivity

rat, mouse, human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MYOG(4656)

Specificity

Myogenin. The epitope is localized to amino acids 158-178.

Immunogen

Recombinant protein from rat myogenin.

Application

Detect Myogenin with Anti-Myogenin Antibody (Mouse Monoclonal Antibody), that has been shown to work in IP, WB, ICC & IHC.
Research Category
Signaling
Research Sub Category
Developmental Signaling
Western blot: 1 μg/mL. Reacts with the ~34 kD protein.

Immunohistochemistry: 1-2 μg/mL on formalin-fixed, paraffin embedded human rhabdomyosarcoma tissue sections.

Immunocytochemistry

Immunoprecipitation: 2 μg/mg

Gel shift assays

Optimal working dilutions must be determined by end user.

Physical form

Format: Purified
Purified immunoglobulin. Liquid in PBS containing 0.08% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sissel Beate Rønning et al.
Differentiation; research in biological diversity, 86(1-2), 13-22 (2013-08-13)
Primary muscle cell model systems from farm animals are widely used to acquire knowledge about muscle development, muscle pathologies, overweight issues and tissue regeneration. The morphological properties of a bovine primary muscle cell model system, in addition to cell proliferation
Peter Genovese et al.
Journal of tissue engineering and regenerative medicine, 15(12), 1131-1143 (2021-09-23)
Volumetric muscle loss (VML) is traumatic or surgical loss of skeletal muscle with resultant functional impairment. Skeletal muscle's innate capacity for regeneration is lost with VML due to a critical loss of stem cells, extracellular matrix, and neuromuscular junctions. Consequences
Rui Chen et al.
Frontiers in cell and developmental biology, 9, 671857-671857 (2021-09-08)
In the present study, the roles of a novel long non-coding RNA (lncRNA), lnc-GD2H, in promoting C2C12 myoblast proliferation and differentiation and muscle regeneration were investigated by quantitative polymerase chain reaction, western blotting, Cell Counting Kit-8, 5-ethynyl-2'-deoxyuridine (EdU), immunofluorescence staining
Renae J Stefanetti et al.
Frontiers in physiology, 5, 30-30 (2014-02-20)
Skeletal muscle atrophy is a critical component of the ageing process. Age-related muscle wasting is due to disrupted muscle protein turnover, a process mediated in part by the ubiquitin proteasome pathway (UPP). Additionally, older subjects have been observed to have
Yaping Nie et al.
Scientific reports, 7, 41608-41608 (2017-02-06)
Differentiation of myoblasts is essential in the development and regeneration of skeletal muscles to form multinucleated, contractile muscle fibers. However, the process of myoblast differentiation in mammals is complicated and requires to be further investigated. In this study, we found

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