MAB1059
Anti-Pigment Epithelium Derived Factor Antibody, clone 10F12.2
clone 10F12.2, Chemicon®, from mouse
Synonym(s):
pigment epithelium-derived factor, proliferation-inducing protein 35, serine (or cysteine) proteinase inhibitor, clade F (alpha-2
antiplasmin, pigment epithelium derived factor), member 1, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin
About This Item
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
10F12.2, monoclonal
species reactivity
human
species reactivity (predicted by homology)
rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SERPINF1(5176)
Related Categories
General description
Specificity
Immunogen
acids 196-347 of human PEDF tagged with GST on the N-terminal was expressed in E. Coli. GST-PEDF is about 46 kDa on SDS-PAGE.
Application
Neuroscience
Neurochemistry & Neurotrophins
Recognizes a protein with a molecular weight of ~46 kDa when tested against immunogen. Western blot positive control is available (Catalog number AG672)
ELISA:
A previous lot of this antibody was used on ELISA.
Immunocytochemistry:
A previous lot of this antibody was used on Immunocytochemistry.
Immunohistochemistry:
A previous lot of this antibody was used on frozen sections.
Optimal working dilutions must be determined by end user.
Quality
Western Blot Analysis:
1:1000 dilution of this lot detected pigment epithelium on 10 μg of human placenta lysate.
Target description
Physical form
Storage and Stability
Analysis Note
Eye tissue.
Other Notes
Legal Information
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recommended
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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