Skip to Content
Merck
All Photos(1)

Key Documents

568520-U

Supelco

Discovery® HS C18 (5 µm) HPLC Columns

L × I.D. 5 cm × 4.6 mm, HPLC Column

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Discovery® HS C18 HPLC Column, 5 μm particle size, L × I.D. 5 cm × 4.6 mm

material

stainless steel column

Agency

suitable for USP L1

product line

Discovery®

feature

endcapped

manufacturer/tradename

Discovery®

packaging

1 ea of

extent of labeling

20% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

5 cm × 4.6 mm

surface area

300 m2/g

surface coverage

3.2 μmol/m2

impurities

<10 ppm metals

matrix

silica gel, high purity, spherical particle platform
fully porous particle

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

120 Å

operating pH range

2-8

application(s)

food and beverages

separation technique

reversed phase

Looking for similar products? Visit Product Comparison Guide

Related Categories

Features and Benefits

  • Stable, low bleed for LC-MS applications
  • Scalable from analytical to preparatory
  • Highly stable to ensure excellent run-to-run and lot-to-lot reproducibility
  • Higher hydrophobicity for better resolution of difficult analytes
   

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
288649-02
288649-01
288143-01
288142-01
288481-02

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Lucie Zelena et al.
Journal of pharmaceutical and biomedical analysis, 137, 70-77 (2017-01-17)
Efavirenz is an antiretroviral drug used in the treatment of HIV-positive patients. A simple, fast and sensitive high-performance liquid chromatography (HPLC) method was developed in order to determine efavirenz in three types of samples provided from pharmacokinetic studies. The analysis
Ismail Khan et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1033-1034, 261-270 (2016-09-07)
A simple, economical, fast, and sensitive RP-HPLC-UV method has been developed for the simultaneous quantification of Sorafenib and paclitaxel in biological samples and formulations using piroxicam as an internal standard. The experimental conditions were optimized and method was validated according
Steinar Uran et al.
Journal of pharmaceutical and biomedical analysis, 44(4), 947-954 (2007-05-08)
Injection of hyperpolarized (13)C-labelled pyruvate ((13)C pyruvate) is under evaluation as an agent for medical metabolic imaging by measuring formation of (13)C lactate using magnetic resonance spectroscopy of the (13)C nuclei. A quantitative method for analysis of these (13)C-labelled substances
Abad Khan et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(20), 1725-1731 (2011-05-10)
A fast, simple, and a reliable high-performance liquid chromatography linked with electrochemical detector (HPLC-ECD) method for the assessment of lipoic acid (LA) and dihydrolipoic acid (DHLA) in plasma was developed using naproxen sodium as an internal standard (IS) and validated
Jolanta Flieger et al.
Journal of separation science, 34(7), 733-739 (2011-03-02)
Highly hydrophilic compounds belonging to biogenic amines were analysed in the reversed-phase system, modified with the addition of ionic liquids: 1-ethyl-3-methyl-imidazolium hexafluorophosphate (EMIM PF(6)) and chaotropic salt NaPF(6) on Discovery HS C18 column at acidic conditions. The effect of the
View All Related Papers

Protocols

Determination of Hydrocortisone from Topical Cream Using Discovery DSC-Si SPE and Reversed-Phase HPLC-UV

Using the method described in this report, an average absolute recovery and RSD value of 99.86 ± 6.99% (n=6) was observed, to determine an average of 1.02% hydrocortisone in topical cream.

Determination of Hydrocortisone from Topical Cream Using Discovery DSC-Si SPE and Reversed-Phase HPLC-UV

Using the method described in this report, an average absolute recovery and RSD value of 99.86 ± 6.99% (n=6) was observed, to determine an average of 1.02% hydrocortisone in topical cream.

Determination of Hydrocortisone from Topical Cream Using Discovery DSC-Si SPE and Reversed-Phase HPLC-UV

Using the method described in this report, an average absolute recovery and RSD value of 99.86 ± 6.99% (n=6) was observed, to determine an average of 1.02% hydrocortisone in topical cream.

Determination of Hydrocortisone from Topical Cream Using Discovery DSC-Si SPE and Reversed-Phase HPLC-UV

Using the method described in this report, an average absolute recovery and RSD value of 99.86 ± 6.99% (n=6) was observed, to determine an average of 1.02% hydrocortisone in topical cream.

Related Content

HPLC Discovery HS C18

HPLC DiscoveryBIO HS C18 products. Available in 3µm, 5µm and 10µm particle sizes.

Chromatograms

application for SPE, application for HPLC

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service