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SAB4200398

Sigma-Aldrich

Anti-TFRC antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-CD71, Anti-TFR, Anti-TFR1, Anti-TR, Anti-TRFR, Anti-Transferrin receptor (p90, CD71), Anti-Transferrin receptor protein 1, Anti-p90, AntiT9

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~90 kDa

species reactivity

mouse, rat, human

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 10-20 μg/mL using rat NRK cells
western blot: 2.5-5.0 μg/mL using whole extracts of human U-2-OS and mouse Neuro-2a cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TFRC(7037)
mouse ... Tfrc(22042)
rat ... Tfrc(64678)

General description

Transferrin receptor (TFRC), also known as p90, CD71, TFR and TFR1, is a transmembrane glycoprotein. It is composed of two disulphide-bonded sub-units, contains three N-linked glycan units and is post-translationally modified with phosphate and fatty acyl groups.

Immunogen

synthetic peptide corresponding to an internal sequence of human TFRC, conjugated to KLH

Application

Anti-TFRC antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Transferrin receptor (TFRC) mediates cellular iron uptake. It binds iron bound transferrin on the cell surface, and the receptor ligand complex undergoes endocytosis via clathrin coated pits. Endosomal acidification leads to iron release, which is transported to the cytosol or to the mitochondria in erythroid cells. The apotransferrin receptor complex is then recycled to the cell surface, where the affinity of apotransferrin for TFRC drops, resulting in its dissociation. TFRC is necessary for development of erythrocytes and the nervous system.

Physical form

solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mando C, et al.
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