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S5393

Sigma-Aldrich

Serum minus IgA/IgM/IgG human

lyophilized powder

Synonym(s):

Depleted Serum

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

human

Quality Level

conjugate

unconjugated

form

lyophilized powder

contains

15 mM sodium azide

packaging

vial of 0.5 mL

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Serum is the liquid component of blood that includes proteins, immunoglobulin and electrolytes. The product can be used for selective removal of contaminating antibodies when immobilized on a solid matrix. This product can also be used in anti-protective antigen (anti-PA) IgG measurements using PA coupled microspheres. Human serum deficient of IgG, IgA, and IgM can react specifically with anti-human whole serum but does not show reactivity with anti-human IgG, IgA, or IgM.

Application

Serum minus IgA/IgM/IgG human has been used as a control serum in immunoelectrophoresis, immunodiffusion,enzyme linked immunosorbent assay. It has also been used as
  • control sera to assess neuraminidase inhibition
  • microneutralization assay
  • single radial hemolysis (SRH) repeatability assay

Quantity

Each vial contains at least 25 mg protein

Reconstitution

Reconstitute with 0.5 mL deionized water.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Marie-Clotilde Bernard et al.
Frontiers in immunology, 13, 909297-909297 (2022-07-06)
Current vaccination strategies against influenza focus on generating an antibody response against the viral haemagglutination surface protein, however there is increasing interest in neuraminidase (NA) as a target for vaccine development. A critical tool for development of vaccines that target
Method for Simultaneous Measurement of Antibodies to 23 Pneumococcal Capsular Polysaccharides
Raymond E. Biagini, Sonela A. Schlottmann
Clinical and Vaccine Immunology : CVI, 10(5), 744-750 (2003)
Claudia M Trombetta et al.
Viruses, 12(1) (2020-01-02)
Influenza D virus is a novel influenza virus, which was first isolated from an ailing swine in 2011 and later detected in cattle, suggesting that these animals may be a primary natural reservoir. To date, few studies have been performed
Validation of Single Radial Haemolysis assay: A reliable method to measure antibodies against influenza viruses
Trombetta CM, et al.
Journal of Immunological Methods, 422, 95-101 (2015)
Age and influenza-specific pre-vaccination antibodies strongly affect influenza vaccine responses in the Icelandic population whereas disease and medication have small effects
Olafsdottir TA, et al.
Frontiers in Immunology, 8, 1872-1872 (2018)

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