Lamp2 (lysosome-associated membrane protein gene)is located in the lysosomal lumen and lysosomal membrane. However upon chronic acidosis in the tumour microenvironment, it is found to be present in the plasma membrane.
Immunogen
a 17 amino acid peptide from near the center of human LAMP-2.
Application
Anti-LAMP-2 antibody has been used in immunofluorescence and western blot.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper)
Biochem/physiol Actions
LAMP2 (lysosome-associated membrane protein gene) preserves lysosomal membranes from acid proteolysis. It serves as an acidity biomarker in spheroids and xenografts. It also has some role in maturation of autophagic vacuoles and cell-cell or cell-extracellular matrix adhesion. Reduction of LAMP2 (lysosome-associated membrane protein gene) can increase acidosis-mediated toxicity. Mutation in LAMP2 can cause cardiomyopathy in young patients.
Linkage
The action of this antibody can be blocked using blocking peptide SBP3627.
Physical form
Solution in phosphate buffered saline containing 0.02% sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
The role and mechanisms of insulin receptor internalization remain incompletely understood. Previous trafficking studies of insulin receptors involved fluorescent protein tagging at their termini, manipulations that may be expected to result in dysfunctional receptors. Our objective was to determine the
Inter-domain tagging implicates caveolin-1 in insulin receptor trafficking and Erk signaling bias in pancreatic beta-cells
Boothe T, et al.
Molecular Metabolism, 5(5), 366-378 (2016)
Bortezomib enhances cancer cell death by blocking the autophagic flux through stimulating ERK phosphorylation
Kao C, et al.
Cell Death & Disease, 5 (2014)
Chronic acidosis in the tumour microenvironment selects for overexpression of LAMP2 in the plasma membrane
Damaghi M, et al.
Nature Communications, 6:8752 (2015)
Effects of white light-emitting diode (LED) exposure on retinal pigment epithelium in vivo
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
