P9625

Phenazine methosulfate

powder, electron acceptor

• Synonym(s): 5-Methylphenazinium methyl sulfate, N-Methylphenazonium methyl sulfate, PMS
• Linear Formula: C₁₃H₁₁N₂ · CH₃SO₄
• CAS Number: 299-11-6
• Molecular Weight: 306.34
• Beilstein: 3898869
• EC Number: 206-072-1
• MDL number: MFCD00011923
• UNSPSC Code: 12352200
• PubChem Substance ID: 24899045
• NACRES: NA.77

Properties

• product name: Phenazine methosulfate,
• form: powder
• λ: 0.01 g/L in H₂O, 1 cm path
• UV absorption: λ: 387.5 nm A_max
• antibiotic activity spectrum: fungi
• Mode of action: DNA synthesis | interferes; cell membrane | interferes
• storage temp.: −20°C
• SMILES string: COS([O-])(=O)=O.C[n+]1c2ccccc2nc3ccccc13
• InChI: 1S/C13H11N2.CH4O4S/c1-15-12-8-4-2-6-10(12)14-11-7-3-5-9-13(11)15;1-5-6(2,3)4/h2-9H,1H3;1H3,(H,2,3,4)/q+1;/p-1
• InChI key: RXGJTUSBYWCRBK-UHFFFAOYSA-M

Description

General description

Phenazines are heterocyclic compounds produced as secondary metabolites by bacteria.

Application

Phenazine methosulfate is used with ascorbic acid to determine nitric oxide reductase activity.
Phenazine methosulfate has been used:

• as a component of succinate dehydrogenase enzyme substrate solution
• to induce superoxide radical generation in red blood cell suspensions and to study its influence on RBC deformability
• as a component of complex II histochemistry media

Biochem/physiol Actions

Phenazine m ethosulfate (PMS) acts as a good electron acceptor. PMS is reduced non-enzymatically by (nicotinamide adenine dinucleotide) NADH and (nicotinamide adenine dinucleotide phosphate) NADPH.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves