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P3369

Sigma-Aldrich

Protamine sulfate

solid, 90.0-110.0% dry basis,≥92% (chromatography), suitable for transduction

Synonym(s):

Salmine sulfate from salmon sperm, Protamine sulfate

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About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.77

product name

Protamine sulfate, meets USP testing specifications

Agency

meets USP testing specifications

Assay

≥92% (chromatography)
90.0-110.0% dry basis

form

solid

mol wt

Mr 5000-10000

technique(s)

transduction: suitable

shipped in

ambient

storage temp.

2-8°C

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General description

Protamine sulfate is a cationic polypeptide having a molecular weight of 5 kDa, isolated from salmon sperm.

Application

Protamine sulfate has been used in the transduction of
  • lentiviruses into human embryonic 293T cells
  • shRNA into mesenchymal stem cells/marrow stromal cells (MSC)
  • lenti Sca1-OCT4 plasmid into human cord blood hematopoietic progenitor CD34+ cells

Biochem/physiol Actions

Alkaline protamine binds to negatively charged heparin. Use of protamine during cardiovascular surgery sometime triggers anaphylactic reactions.
Protamine facilitates direct transfection of microbial cells, and of human cells using cationic liposomes. Protamine effectively neutralizes the anticoagulation effects of heparin.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A polycationic peptide, protamine sulfate, USP, has been shown to be able to condense plasmid DNA efficiently for delivery into several different types of cells in vitro by several different types of cationic liposomes. The monovalent cationic liposomal formulations (DC-Chol
Reprogramming of Human Cord Blood CD34+ Cells into Induced MSCs
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Rosova I, et al.
Tissue Engineering: Part A, 16(8), 2627-2639 (2010)

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