P0501

Phenolphthalein β-D-glucuronide

β-glucuronidase substrate

• Synonym(s): Phenolphthalein β-D-glucosiduronic acid, Phenolphthalein glucuronic acid, Phenolphthalein mono-β-D-glucosiduronic acid
• Empirical Formula (Hill Notation): C₂₆H₂₂O₁₀
• CAS Number: 15265-26-6
• Molecular Weight: 494.45
• Beilstein: 68526
• MDL number: MFCD00135881
• UNSPSC Code: 12352204
• PubChem Substance ID: 24898108
• NACRES: NA.32

Properties

• Assay: ≥97% (TLC)
• form: powder
• solubility: H₂O: soluble 100 mg/mL
• storage temp.: −20°C
• SMILES string: O[C@@H]1[C@@H](O)[C@@H](O[C@@H]([C@H]1O)C(O)=O)Oc2ccc(cc2)C3(OC(=O)c4ccccc34)c5ccc(O)cc5
• InChI: 1S/C26H22O10/c27-15-9-5-13(6-10-15)26(18-4-2-1-3-17(18)24(33)36-26)14-7-11-16(12-8-14)34-25-21(30)19(28)20(29)22(35-25)23(31)32/h1-12,19-22,25,27-30H,(H,31,32)/t19-,20-,21+,22-,25+,26?/m0/s1
• InChI key: FXJYOZKDDSONLX-XADSOVDISA-N

Description

Application

β-glucuronidase activity was determined by the amount of phenolphthalein released from 0.01 M henolphthalein β-D-glucuronide.

Phenolphthalein β-D-glucuronide has been used as a substrate for the detection of β-D-glucuronidase activity in fecal bacteria.

Biochem/physiol Actions

Phenolphthalein β-D-glucuronide is a chromogenic substrate for β-glucuronidase enzyme and the resultant colored product of the reaction is measured at 552 nm.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves