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MAK004

Sigma-Aldrich

Glutamate Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

L-Glutamate Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 100 colorimetric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric

relevant disease(s)

neurological disorders; cancer

storage temp.

−20°C

General description

Glutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism.[1] The synthesis of α-KG from glutamate by glutamate dehydrogenase is a key TCA cycle anaplerotic reaction. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen.[2] Within the mammalian nervous system, glutamate is the most abundant excitatory neurotransmitter.[1] It is believed to be involved in learning and memory and may also be involved in diseases like amyotrophic lateral sclerosis, autism, some forms of mental retardation, and Alzheimer′s disease.[3] Glutamate is present in a wide variety of foods, and has been used as a flavor enhancer in food industry.

Application

Glutamate Assay Kit has been used to determine the concentration of glutamate in cerebrospinal fluid in a rat subarachnoid hemorrhage model.[4] It has also been used to determine cytosolic and secreted glutamate concentrations in cerebrospinal fluid of patients with amyotrophic lateral sclerosis.[5]

Suitability

Suitable to determine glutamate concentration in a variety of samples.

Principle

Glutamate concentration is determined by an enzymatic assay, which results in a colorimetric (450 nm) product, proportional to the glutamate present.

Pictograms

Health hazardCorrosion
GHS08,GHS05

Signal Word

Danger

Hazard Statements

H314,H334

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8K20K06290
8A17K06290
8D08K06290
8H25K06290
7C26K06290

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Developing medications targeting glutamatergic dysfunction in autism: progress to date.
Fung L K and Antonio Y H
CNS Drugs, 29(6), 453-463 (2015)
Cheng Zhang et al.
Translational stroke research, 11(4), 799-811 (2019-12-14)
The blood-brain barrier (BBB) disruption leads to the vasogenic brain edema and contributes to the early brain injury (EBI) after subarachnoid hemorrhage (SAH). However, the mechanisms underlying the BBB damage following SAH are poorly understood. Here we reported that the
Leena S Knight et al.
Neurochemical research, 42(12), 3490-3503 (2017-09-18)
Betaine (N-trimethylglycine), a common osmolyte, has received attention because of the number of clinical reports associating betaine supplementation with improved cognition, neuroprotection and exercise physiology. However, tissue analyses report little accumulation of betaine in brain tissue despite the presence of
Glutamate as a neurotransmitter in the healthy brain.
Zhou Y and Danbolt N C
Journal of Neural Transmission. General Section, 121(8), 799-817 (2014)
J Jašprová et al.
Scientific reports, 8(1), 7444-7444 (2018-05-12)
Phototherapy was introduced in the early 1950's, and is the primary treatment of severe neonatal jaundice or Crigler-Najjar syndrome. Nevertheless, the potential biological effects of the products generated from the photodegradation of bilirubin during phototherapy remain unknown. This is very
View All Related Papers

Articles

Glutamine Metabolism in Cancer Cells

Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.

Glutamine Metabolism in Cancer Cells

Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.

Glutamine Metabolism in Cancer Cells

Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.

Glutamine Metabolism in Cancer Cells

Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.

Questions

1–5 of 5 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. このキットで定量できるグルタミン酸の濃度の範囲はどれくらいでしょうか？ また、1キットあたり何サンプル分の測定が可能でしょうか？

    1 answer

    1. The Glutamate Assay Kit (MAK004) has a lower limit of detection of 40 µM and shows a linear standard curve up to 200 µM glutamate. One kit is designed to measure up to 100 samples.

      Helpful?

  4. What is the reason for the lack of color change in the samples at 100 mM concentration, despite the expectation of a rapid or strong color change if the glutamate concentration is out of the standard range?

    1 answer

    1. The standard prepared at 10 nmole/well corresponds to 200 uM. To calculate this, you can use the formula M1V1 = M2V2 based on the preparation in the bulletin. The highest point standard provided in the kit is 200 uM. If the concentration exceeds the range of the standard curve, extrapolating the information for a decision is not appropriate. Dilution is necessary to ensure the concentration is within the standard curve for a correct determination of the glutamate concentration. A concentration of 100 mM is significantly out of range and may be causing the "Hook effect," leading to no color development. The "Hook effect" occurs due to the saturation of the reagents involved in the reaction for color production.

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  5. Why are cloudy white precipitates sometimes observed after reconstituting the enzyme mix in the Glutamate Assay Kit (MAK004)? Is there a way to reconstitute it?

    1 answer

    1. The presence of precipitates in the enzyme mix may be attributed to the reconstitution temperature. It is suggested to ensure complete equilibration of the Assay Buffer at room temperature before use. Reconstitute the Enzyme Mix according to the protocol using 220 µL Assay Buffer and keep it on ice during the assay. Gently vortexing is also an option. It is noted that the precipitates in the Enzyme Mix should not affect the assay, given that the mix is more concentrated than necessary. Care should be taken to avoid pipetting the precipitates into the wells. However, occasional precipitates in the Enzyme Mix should not have a detrimental impact.

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