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L4277

Sigma-Aldrich

Lipase from Aspergillus oryzae

≥20,000 U/g

Synonym(s):

Palatase® 20,000L

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized

specific activity

≥20,000 U/g

storage temp.

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

General description

Lipase from Aspergillus Oryzae is a carboxylesterase and belongs to the α/β-hydrolase family. It comprises a lid domain, hinge domain and a catalytic triad Ser?His?Asp/Glu. The three-dimensional structure is a α/β hydrolase fold which is very similar as that of esterase enzyme.

Application

Lipase from Aspergillus Oryzae has been used:

  • in the partial digestion of triacylglycerols (TAG)
  • in the digestion of (12-ricinoleoylricinoleoyl)diricinoleoylglycerol (RRRR) present in castor oil
  • in the hydrolysis of linseed oil

Biochem/physiol Actions

Lipase catalyzes the hydrolysis of monoacylglycerols and diacylglycerols and its activity is inhibited by divalent actions. Aspergillus Oryzae lipase is an industrial enzyme with application in food, detergent and pharmaceutical industries. It is also used in immobilization studies for 1,3-dioleoyl-2-palmitoylglycerol (OPO) synthesis. Aspergillus Oryzae lipase may have potential in producing biodiesel from waste cooking oil.

Preparation Note

purified 1,3-specific lipase from Aspergillus Oryzae produced by submerged fermentation of a genetically modified Aspergillus Oryzae microorganism

Legal Information

A product of Novozyme corp.
Palatase is a registered trademark of Novozymes Corp.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Thais de Andrade Silva et al.
Scientific reports, 12(1), 6815-6815 (2022-04-28)
The use of enzymes immobilized on nanomagnetic supports has produced surprising results in catalysis, mainly due to the increase in surface area and the potential for recovery and reuse. However, the meticulous control of the process and difficulties in reproducibility
Purification and characterization of a lipase from Aspergillus oryzae
Toida J, et al.
Bioscience, Biotechnology, and Biochemistry, 59(7), 1199-1203 (1995)
Xianming Zhao et al.
Frontiers in microbiology, 10, 645-645 (2019-04-12)
Thraustochytrium is a marine protist that can accumulate a large amount of very long chain polyunsaturated fatty acids (VLCPUFA) in triacylglycerols (TAG). How these freshly synthesized VLCPUFAs are channeled into TAG remains unknown. In this study, the glycerolipid profile of
Jiann-Tsyh Lin et al.
Journal of agricultural and food chemistry, 56(10), 3616-3622 (2008-04-30)
(12-Ricinoleoylricinoleoyl)diricinoleoylglycerol (RRRR), a tetraacylglycerol, was identified earlier in castor oil. Using ESI-MS (4), 95% of the 12-ricinoleoylricinoleoyl chain was identified at the sn-2 position of the glycerol backbone of RRRR. Regiospecific location of the 12-ricinoleoylricinoleoyl chain of RRRR on the
Preliminary Investigation: Stearidonic Acid Production by Genetically Modified Saccharomyces cerreviseae Using Linseed Oil as A Fatty Acid Source
Muzakhar K
Jurnal Ilmu Dasar, 9(1), 8-Jan-8-Jan (2008)

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