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Key Documents

L1516

Sigma-Aldrich

Lectin from Wisteria floribunda

buffered aqueous solution, biotin conjugate

Synonym(s):

Wisteria floribunda agglutinin, WFA

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About This Item

MDL number:
MFCD00166187
UNSPSC Code:
12352202
NACRES:
NA.32

conjugate

biotin conjugate

Quality Level

200

form

buffered aqueous solution

packaging

pkg of 2 mg active conjugate

storage temp.

2-8°C

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Biochem/physiol Actions

WFA is not blood group specific, but has an affinity for N-acetyl-D-galactosamine.

Packaging

Package size based on active conjugate content

Preparation Note

Protein is biotinylated utilizing aminocaproyl spacer.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hr incubation at 25 °C.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Marie Mennesson et al.
eNeuro, 7(4) (2020-08-14)
NETO2 is an auxiliary subunit for kainate-type glutamate receptors that mediate normal cued fear expression and extinction. Since the amygdala is critical for these functions, we asked whether Neto2 -/- mice have compromised amygdala function. We measured the abundance of
Margaret Po-Shan Luke et al.
Molecular and cellular neurosciences, 107, 103527-103527 (2020-07-08)
Vision loss has long since been considered irreversible after a critical period; however, there is potential to restore limited vision, even in adulthood. This phenomenon is particularly pronounced following complete loss of vision in the dominant eye. Adult neural cell
Carly M Drzewiecki et al.
Brain structure & function, 225(8), 2495-2507 (2020-09-12)
The prefrontal cortex (PFC) is a late developing region of the cortex, and its protracted maturation during adolescence may confer a period of plasticity. Closure of critical, or sensitive, periods in sensory cortices coincides with perineuronal net (PNN) expression, leading
Kelly E Carstens et al.
The European journal of neuroscience, 53(12), 4005-4015 (2020-11-22)
Current methods of experimentally degrading the specialized extracellular matrix (ECM), perineuronal nets (PNNs) have several limitations. Genetic knockout of ECM components typically has only partial effects on PNNs, and knockout of the major ECM component aggrecan is lethal in mice.
Soledad Domínguez et al.
Cell reports, 29(5), 1099-1112 (2019-10-31)
Adolescence is a vulnerable period characterized by major cognitive changes. The mechanisms underlying the emergence of new cognitive functions are poorly understood. We find that a long-term depression of inhibitory transmission (iLTD) from parvalbumin-expressing (PV+) interneurons in the hippocampal area Cornu
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