E8140
Elastase from human leukocytes
lyophilized powder, ≥50 units/mg protein (Bradford)
Synonym(s):
Lysosomal elastase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
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biological source
human leucocytes
Quality Level
form
lyophilized powder
specific activity
≥50 units/mg protein (Bradford)
mol wt
29 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... ELA2(1991) , ELANE(1991)
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General description
Elastase is a proteolytic enzyme. It is a member of the subgroup named, peptidyl peptide hydrolases. It is a major anatomic constituent of arteries. It is mainly found in the pancreas and pancreatic juice of various birds and mammals. It is also present in human serum, granulocytes and erythrocytes.
Application
Elastase from human leukocytes has been used:
- to measure serum elastase activity
- in proteolytic digestion of fibronectin and salivary glands
- in neutrophil elastase (NE) activity assay
- cell-free NE digestion of E-cadherin
- scratch wound assay
- in a study that determined that fragments of Nle3-angiotensin(1-7) accelerate healing in dermal models
Biochem/physiol Actions
Elastase enzyme is capable of releasing soluble peptides from insoluble elastin fibers with the help of a proteolytic process. It can stimulate disintegration of the axoneme with the help of adenosine triphosphate (ATP). Unlike pancreatic elastase the leukocyte enzyme has a preferential cleavage for the carboxyl side of valine, but will also cleave to a lesser extent after alanine. Natural substrates include elastin, cartilage proteoglycans, collagen types I, II, II and IV, and fibronectin.
Physical properties
Leukocyte elastase is a 29 kDa serine endoprotease of the Proteinase S1 Family. It exists as a single 238 amino acid-peptide chain with four disulfide bonds. It contains two or thee N-linked glycans of variable composition which account for its three major isoforms.
Isoelectric point: pI = 8.77 - 9.55
Isoelectric point: pI = 8.77 - 9.55
Unit Definition
One unit will release one nanomole of p-nitrophenol per sec from BOC-L-alanine p-nitrophenyl ester at pH 6.5 at 37 °C.
Physical form
Lyophilized from 0.05 M sodium acetate (pH 5.5) and 0.6 M NaCl
Application
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Description
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inhibitor
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Description
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substrate
Product No.
Description
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Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Emily J Meyer et al.
Protein science : a publication of the Protein Society, 29(12), 2495-2509 (2020-10-22)
Corticosteroid-binding globulin (CBG) transports cortisol and other steroids. High-affinity CBG (haCBG) undergoes proteolysis of the reactive center loop (RCL) by neutrophil elastase (NE) altering conformation to low-affinity CBG (laCBG). Elevated temperature reduces CBG:cortisol binding affinity. Surface plasmon resonance was used
Proteolytic Processing of the Epithelial Adherens Junction Molecule E-Cadherin by Neutrophil Elastase Generates Short Peptides With Novel Wound-Healing Bioactivity
Gordon MH, et al.
Cellular and molecular gastroenterology and hepatology, 7(2), 483-483 (2019)
Yiming Shao et al.
Journal of cellular and molecular medicine, 26(7), 2089-2103 (2022-02-12)
Neutrophils release neutrophil extracellular traps (NETs) to capture and kill pathogens, but excessive NET release can damage the surrounding tissues. Myeloperoxidase (MPO) and neutrophil elastase (NE) are thought to be important in promoting histone depolymerization and DNA breakage in the
Maurits L van Montfoort et al.
Arteriosclerosis, thrombosis, and vascular biology, 33(1), 147-151 (2012-10-30)
The formation of neutrophil extracellular traps and the exposure of nucleosomes on these neutrophil extracellular traps contribute to coagulation activation and the propagation of deep vein thrombosis (DVT) in animal models. However, no data are available on the role of
Benjamin J Staudinger et al.
American journal of respiratory and critical care medicine, 189(7), 812-824 (2014-01-29)
Progress has been made in understanding how the cystic fibrosis (CF) basic defect produces lung infection susceptibility. However, it remains unclear why CF exclusively leads to chronic infections that are noninvasive and highly resistant to eradication. Although biofilm formation has
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