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D8764

Sigma-Aldrich

Deoxyribonuclease II from bovine spleen

Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein

Synonym(s):

DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase

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About This Item

CAS Number:
9025-64-3
Enzyme Commission number:
3.1.22.1 (BRENDA, IUBMB)
EC Number:
232-801-8
MDL number:
MFCD00130919
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bovine spleen

type

Type V

form

essentially salt-free, lyophilized powder

specific activity

≥1,000 units/mg protein

mol wt

38 kDa

concentration

≥50% protein

technique(s)

DNA extraction: suitable
tissue culture: suitable

suitability

suitable for molecular biology

application(s)

cell analysis

foreign activity

RNase ≤0.1%

storage temp.

−20°C

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Application

Deoxyribonuclease II from bovine spleen has been used:
  • as a component of digestion mix to isolate immune cells from colon samples
  • to digest retinal explants for single-cell suspension preparation
  • for isolation of stromal cells from human colon tissue
  • in the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart

Biochem/physiol Actions

Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3′-phosphates. in vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.

Unit Definition

One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25 °C; [Mg2+] = 0.83 mM

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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K Nitta et al.
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 114(2), 119-128 (1994-02-01)
Deoxyribonucleases from eggs and the liver of Xenopus laevis were partially purified by DEAE-cellulose and heparin-Sepharose affinity column chromatographies. The fractions having egg and liver DNase activities were eluted on high performance liquid chromatography through TSK gel G3000SW at the
Dopamine D2 receptor stimulation differentially affects voltage-activated calcium channels in rat pituitary melanotropic cells.
Keja J A, et al.
The Journal of Physiology, 450(1), 409-435 (1992)
S Ingebrandt et al.
Biosensors & bioelectronics, 16(7-8), 565-570 (2001-09-07)
An extracellular recording system has been designed for the detection of electrical cell signals using p-channel or n-channel field-effect transistor (FET) arrays with non-metallized gates. Signals from rat heart muscle cell were recorded by these devices and the results described
STUDIES ON ACID DEOXYRIBONUCLEASE. II. ISOLATION AND CHARACTERIZATION OF SPLEEN-ACID DEOXYRIBONUCLEASE.
G BERNARDI et al.
Biochemistry, 3, 1419-1426 (1964-10-01)
Therese Ostberg et al.
Arthritis and rheumatism, 62(10), 2963-2972 (2010-06-10)
High mobility group box chromosomal protein 1 (HMGB-1) is a DNA binding nuclear protein that can be released from dying cells and activated myeloid cells. Extracellularly, HMGB-1 promotes inflammation. Clinical and experimental studies demonstrate that HMGB-1 is a pathogenic factor
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