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D4031

Sigma-Aldrich

Dulbecco′s Phosphate Buffered Saline

liquid, Modified, with 36 mg sodium pyruvate, 50 mg streptomycin sulfate, 100 mg kanamycin monosulfate, 1000 mg glucose/L and CaCl₂, liquid, 0.1 μm filtered, suitable for cell culture

Synonym(s):

DPBS

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About This Item

MDL number:
MFCD00131855
UNSPSC Code:
12352207
eCl@ss:
32129211
NACRES:
NA.71

product name

Dulbecco′s Phosphate Buffered Saline, Modified, with 36 mg sodium pyruvate, 50 mg streptomycin sulfate, 100 mg kanamycin monosulfate, 1000 mg glucose/L and CaCl2, liquid, 0.1 μm filtered, suitable for cell culture

sterility

0.1 μm filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: 0.036 g/L
glucose: 1.0 g/L

shipped in

ambient

storage temp.

2-8°C

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General description

Dulbecco′s phosphate buffered saline is a balanced salt solution, and a fully supplemented isotonic solution. It contains antibiotics and glucose and inorganic ions as energy sources. It is frequently used with primary cells where risk of contamination may be high.

Application

Dulbecco′s phosphate buffered saline has been used :
  • To study how ultrasound could enhance the delivery of anti inflammatory drug through cornea in corneal infections.
  • In the detection of microtubules in sludge worms.
  • For recovering bone marrow cells from the femur of green fluorescent protein (GFP) expressing mice.
  • To study the inhibitory effect of 3-methyladenine on autophagy, in normal rat kidney (NRK) cell expressing GFP-LC3 (autophagy marker).

Formula variant

Contains 50 mg/L streptomycin sulfate and 100 mg/L kanamycin monosulfate.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Zachary DeLoughery et al.
Chemical research in toxicology, 27(5), 843-851 (2014-03-22)
Cr(VI) genotoxicity is caused by products of its reductive metabolism inside the cells. Reactive oxygen species (ROS) and Cr(V,IV) intermediates are potential sources of oxidative damage by Cr(VI). Here, we investigated seven fluorescent probes for the detection of ROS and
Ultrasound-enhanced delivery of antibiotics and anti-inflammatory drugs into the eye
Nabili M, et al.
Ultrasound in Medicine & Biology, 39(4), 638-646 (2013)
Transplanted bone marrow cells do not provide new oocytes but rescue fertility in female mice following treatment with chemotherapeutic agents
Santiquet N, et al.
Cellular Reprogramming, 14(2), 123-129 (2012)
The ovary of tubifex tubifex (clitellata, naididae, tubificinae) is composed of one, huge germ-line cyst that is enriched with cytoskeletal components
Urbisz AZ, et al.
PLoS ONE, 10(5) (2015)
C Tonus et al.
Theriogenology, 88, 197-206 (2016-10-19)
In the present study, we compare a classical slow freezing (SLF) method and an aseptic vitrification (Vitrif) technique to cryopreserve a stable primordial germ cell (PGCs) line issued from the Ardennaise chicken breed. Viability immediately after warming was close to
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