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Key Documents

D2376

Sigma-Aldrich

Deoxyribonucleic acid methyl green

DNAse substrate

Synonym(s):

Deoxyribonucleic acid–methyl green complex, DNA-Methyl Green

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About This Item

MDL number:
UNSPSC Code:
12352204
NACRES:
NA.32

form

solid

storage temp.

−20°C

General description

Methyl green is a widely used nuclei counterstain for histochemical methods. Methyl green binds to the DNA major groove and is used for tracking triplex formation. The positive charge on the aniline rings in the methyl green enables its strong binding to DNA. Methyl green aids clear visualization in confocal laser scanning microscopy.

Application

Deoxyribonucleic acid methyl green has been used in DNA methyl green bioassay.

Substrates

Substrate for DNase

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos
Prieto D, et al.
Journal of Visualized Experiments, 315(99) (2015)
Synthesis, DNA-binding, and Antiviral Activity of Certain Pyrazolo [3, 4-d] pyrimidine Derivatives
El-Bendary ER and Badria FA
Arch. Pharm. (Weinheim), 333(4), 99-103 (2000)
Methyl green: A DNA major-groove binding drug
Kim SK and Norden B
Febs Letters, 315(1), 61-64 (1993)
Cytotoxic and DNA interaction activities of extracts from medicinal plants used in Argentina
Mongelli E, et al.
Journal of Ethnopharmacology, 71(1-2), 145-151 (2000)
Simultaneous detection of DAB and methyl green signals on apoptotic nuclei by confocal laser scanning microscopy
Itoh J, et al.
Acta Histochemica Et Cytochemica, 36(4), 367-376 (2003)

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