CS1010
Histone Deacetylase Assay Kit, Fluorometric
sufficient for 100 assays (96 well plates)
Synonym(s):
HDAC Assay Kit
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About This Item
Recommended Products
usage
sufficient for 100 assays (96 well plates)
Quality Level
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... HDAC1(3065)
Application
The Histone Deacetylase Assay Kit provides a simple method for the detection of HDAC activity based on a two-step enzymatic reaction. The substrate for this enzymatic assay is a substituted peptide with an acetylated lysine residue and a bound fluorescent group. The first step of the reaction is deacetylation of the acetylated lysine side chain by the HDAC containing sample (HeLa cell extract, purified enzyme, etc.). The second step is the cleavage of the deacetylated substrate by the developer solution and the release of the free highly fluorescent group. The measured fluorescence is directly proportional to the deacetylation activity of the sample.
Biochem/physiol Actions
Histone deacetylases (HDACs) are enzymes that remove acetyl groups from histone proteins. HDACs are important regulators of gene expression and genome function. They are implicated in a number of human disease states, namely several cancers, neurological disorders, and aging. Therefore, HDAC are relevant key targets for therapeutic intervention. Moreover, histone deacetylase inhibitors have been shown to serve as anti-tumor agents.
Features and Benefits
The kit includes all the reagents required for the fast and easy measurement of HDAC activity in cell or nuclear extracts, or with purified enzyme preparations. In addition, the kit provides HeLa cell lysate as a source of HDAC activity for inhibitor screening or as a positive control, a HDAC inhibitor (Trichostatin A), and a standard to enable activity quantitation.
Analysis Note
The Histone Deacetylase Assay Kit has been tested on HeLa, HEK 293T, NIH 3T3, and U 973 cell extracts.
Kit Components Only
Product No.
Description
- Assay Buffer 20 mL
- Developer Solution 1.5 mL
- HDAC Inhibitor (Trichostatin A), 1 mM 50 μL
- HDAC Substrate, 20 mM 50 μL
- HeLa Cell Lysate 500 μL
- Standard (non-acetylated substrate), 4 mM 50 μL
Storage Class Code
10 - Combustible liquids
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
Certificates of Analysis (COA)
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Bartosz Bieszczad et al.
Pharmaceuticals (Basel, Switzerland), 14(9) (2021-09-29)
Histone deacetylase (HDAC) inhibitors are a class of drugs used in the cancer treatment. Here, we developed a library of 19 analogues of Vorinostat, an HDAC inhibitor used in lymphomas treatment. In Vorinostat, we replaced the hydrophobic phenyl group with
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Journal of natural products, 77(7), 1753-1757 (2014-07-08)
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The Journal of biological chemistry, 291(36), 18765-18777 (2016-07-16)
The ubiquitin/proteasome pathway is a well characterized system for degrading intracellular proteins, although many aspects remain poorly understood. There is, for instance, a conspicuous lack of understanding of the site(s) where nuclear proteins are degraded because the subcellular distribution of
Ze Yang et al.
Cell research, 20(3), 276-287 (2010-01-27)
LSD1 (KDM1 under the new nomenclature) was the first identified lysine-specific histone demethylase belonging to the flavin-dependent amine oxidase family. Here, we report that AOF1 (KDM1B under the new nomenclature), a mammalian protein related to LSD1, also possesses histone demethylase
Jason D Plummer et al.
eLife, 10 (2021-03-31)
Methionine restriction (MR) dramatically extends the healthspan of several organisms. Methionine-restricted rodents have less age-related pathology and increased longevity as compared with controls, and recent studies suggest that humans might benefit similarly. Mechanistically, it is likely that the decreased IGF-1
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