CL4B200
Sepharose™ CL-4B Size Exclusion Resin
Cross-linked, 90-350 mesh
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About This Item
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product name
Sepharose™ CL-4B, Cross-linked
Quality Level
form
beads
technique(s)
LPLC: suitable
matrix
Agarose
matrix active group
polymer
bead diameter
40-165 μm
pore size
30,000-5,000,000 fractionation range (Dextrans)
60,000-20,000,000 fractionation range (Globular proteins)
separation technique
size exclusion (SEC)
storage temp.
2-8°C
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General description
CL4B200-1L′s update product number is GE17-0150-01
Application
- Molecular size distribution assessment of Haemophilus influenzae vaccine containing lactose by HPAEC-PAD and colorimetric assays: This study used Sepharose CL-4B in a methodology assessing molecular size distribution in vaccine formulations, indicating its application in quality control and development processes in vaccine production (Nadim AH et al., 2022).
- Camel hydatidosis diagnostic kit: optimization of turnip and horseradish peroxidase conjugates using glutaraldehyde method: This research mentions the use of Sepharose CL-4B in the development of diagnostic kits, suggesting its role in enhancing diagnostic assay development through effective enzyme conjugation techniques (Kandil OM et al., 2020).
- Pectin from leaves of birch (Betula pendula Roth.): Results of NMR experiments and hypothesis of the RG-I structure: Sepharose CL-4B was utilized in the structural elucidation of pectins from birch leaves, underscoring its importance in the structural study of polysaccharides which is essential for understanding their biological functions and industrial applications (Golovchenko VV et al., 2022).
Legal Information
Sepharose is a trademark of Cytiva
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 3
Storage Class Code
3 - Flammable liquids
WGK
WGK 1
Flash Point(F)
100.4 - 109.4 °F
Flash Point(C)
38 - 43 °C
Certificates of Analysis (COA)
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Martin Devenport et al.
Biochemistry, 45(31), 9540-9549 (2006-08-02)
The gene Aedes aegypti intestinal mucin 1 (AeIMUC1) encodes a putative peritrophic matrix (PM) protein that is expressed in the midgut of mosquito larvae and adults and is upregulated in response to exposure to heavy metals. The AeIMUC1 protein has
Alberta N A Aryee et al.
Applied biochemistry and biotechnology, 168(8), 2105-2122 (2012-10-24)
Grey mullet (Mugil cephalus) lipase was isolated using para-aminobenzamidine agarose and immobilized on octyl Sepharose CL-4B (o-Sep). Immobilized grey mullet lipase (GMLi) had a 10 °C higher optimum temperature compared to the free enzyme and showed remarkable thermal stability. GMLi was
M Downham et al.
Journal of chromatography, 584(1), 59-67 (1992-12-11)
The constant region of human kappa light chain (Ck) was linked to Escherichia coli beta-galactosidase, using standard molecular cloning techniques. The binding of Ck-beta-galactosidase fusions to a number of different murine monoclonal antibodies, specific for Ck, was improved by the
C Srisawat et al.
Nucleic acids research, 29(2), E4-E4 (2001-01-05)
Sephadex-binding RNA ligands (aptamers) were obtained through in vitro selection. They could be classified into two groups based on their consensus sequences and the aptamers from both groups showed strong binding to Sephadex G-100. One of the highest affinity aptamers
S Cochet et al.
Journal of chromatography. A, 663(2), 175-186 (1994-03-11)
A range of substances were screened to find eluents for human immunoglobulin G (IgG) which are retained with a strong affinity by immobilized Drimarene Rubine R/K-5BL. The strong affinity of IgG for the dye is partly due to the presence
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