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C9243

Sigma-Aldrich

Anti-CHIP (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-CHIP, carboxy terminus of Hsp70p-interacting protein, Anti-HSPABP2, Anti-NY-CO-7, Anti-STUB1, stip2 homology and U-box containing protein 1, Anti-UBOX1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~35 kDa

species reactivity

human, mouse, rat

concentration

~1 mg/mL

technique(s)

western blot: 1-2 μg/test using HEK-293T cells lysate and mouse brain extract (S2 fraction)

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... STUB1(10273)
mouse ... Stub1(56424)
rat ... Stub1(287155)

General description

CHIP (carboxy terminus of Hsp70-interacting protein, also known as STIP1-homology and U-box containing protein 1, STUB1, HSPABP2, NY-CO-7, SDCCAG7, UBOX1), is a dual-function chaperone/E3 ubiquitin ligase. CHIP has 3 functional domains: a N-terminal tetratricopeptide repeat (TPR), a U-box at its C-terminus, and a highly charged internal region. It is located on human chromosome 16p13.3.

Application

Anti-CHIP (C-terminal) antibody produced in rabbit has been used in western blotting and histology to stain testes and ovary sections.
Anti-CHIP (C-terminal) antibody produced in rabbit is suitable for immunoblotting at a working concentration of 1-2 μg/mL using HEK-293T cells lysate and mouse brain extract (S2 fraction).

Biochem/physiol Actions

CHIP (carboxy terminus of Hsp70-interacting protein) plays a crucial role in regulating protein quality control at multiple levels. It ubiquitinates Hsp70, a cytosolic chaperone and enhances refolding of stress damaged proteins. Additionally, it has E3 ubiquitin ligase activity and triggers proteasome degradation of irreversibly damaged proteins to prevent cellular toxicity. The ubiquitination, aggregation and degradation of several proteins involved in neurodegenerative disorders including tau, huntingtin, Cu/Zn SOD1, ataxin-1, and α-synuclein are regulated by CHIP and Hsp70. The aggregation and toxicity of polyglutamine (polyQ) expanded proteins is suppressed by CHIP. It also regulates stress-dependent apoptosis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yasaman Pakdaman et al.
International journal of molecular sciences, 22(11) (2021-06-03)
Variants in STUB1 cause both autosomal recessive (SCAR16) and dominant (SCA48) spinocerebellar ataxia. Reports from 18 STUB1 variants causing SCA48 show that the clinical picture includes later-onset ataxia with a cerebellar cognitive affective syndrome and varying clinical overlap with SCAR16.
Cloning and characterization of carboxyl terminus of heat shock cognate 70-interacting protein gene from the silkworm, Bombyx mori
Ohsawa T, et al.
Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 201, 29-36 (2016)
Bridget F Donnelly et al.
The Journal of biological chemistry, 288(18), 13124-13135 (2013-03-14)
The thiazide-sensitive NaCl cotransporter (NCC) is the primary mediator of salt reabsorption in the distal convoluted tubule and is a key determinant of the blood pressure set point. Given its complex topology, NCC is inefficiently processed and prone to endoplasmic
Ataxia and hypogonadism caused by the loss of ubiquitin ligase activity of the U box protein CHIP
Shi CH, et al.
Human Molecular Genetics, 23(4), 1013-1024 (2013)
Leonard Petrucelli et al.
Human molecular genetics, 13(7), 703-714 (2004-02-14)
Molecular chaperones, ubiquitin ligases and proteasome impairment have been implicated in several neurodegenerative diseases, including Alzheimer's and Parkinson's disease, which are characterized by accumulation of abnormal protein aggregates (e.g. tau and alpha-synuclein respectively). Here we report that CHIP, an ubiquitin

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