C9017

Concanavalin A from Canavalia ensiformis (Jack bean)

Sepharose^™ conjugate, buffered aqueous suspension

• Synonym(s): ConA
• UNSPSC Code: 12161501
• NACRES: NA.32

Properties

• biological source: Canavalia ensiformis
• conjugate: Sepharose^™ conjugate
• form: buffered aqueous suspension
• contains: 20% ethanol as preservative
• extent of labeling: 10-16 mg per mL
• matrix: Sepharose 4B
• matrix activation: cyanogen bromide
• matrix attachment: amino
• matrix spacer: 1 atom
• capacity: ≥25 mg/mL binding capacity (thyroglobulin)
• storage temp.: 2-8°C

Description

Application

Used for the purification of glycoproteins containing mannose.

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca²⁺ and Mn²⁺ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Physical form

Suspension in 0.1 M acetate buffer, pH 6.0, containing 1 M NaCl, and 1 mM each of CaCl₂ , MgCl₂, and MnCl₂

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca²⁺ and Mn²⁺ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Legal Information

Sepharose is a trademark of Cytiva

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317,H334,H361
• Precautionary Statements: P202 - P261 - P272 - P280 - P302 + P352 - P308 + P313
• Hazard Classifications: Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 102.2 °F
• Flash Point(C): 39 °C