BCA1

Bicinchoninic Acid Kit for Protein Determination

for 200-1000 μg/ml protein

• Synonym(s): BCA protein assay
• UNSPSC Code: 12352106
• NACRES: NA.32

Properties

• usage: sufficient for ~500 assays
• storage temp.: 2-8°C

Description

Application

Bicinchoninic Acid Kit has been used for the protein estimation in salivary proteome samples, human embryonic kidney 293 cells and luteal cells.

Features and Benefits

• Simple, sensitive colorimetric assay for proteins
• Two stable reagents are used for the working solution
• Faster and easier than the Lowry protein assay
• Compatible with many detergents both ionic and nonionic
• Less variation between different proteins than the Bradford dye-binding assay
• Adaptable for use in microwell plates

Principle

Proteins reduce alkaline Cu(II) to Cu(I) in a concentration-dependent manner. Bicinchoninic acid is a highly specific chromogenic reagent for Cu(I), forming a purple complex with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration. This is an alternative to the Folin-Ciocalteu reagent for protein determination.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Kit Components Only

• Bicinchoninic Acid Solution 1 L
• 4%(w/v) CuSO_{4 ·} 5H₂O Solution 25 mL
• Protein Standard Solution 5 x 1

Safety Information

• Pictograms: GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H290,H318,H410
• Precautionary Statements: P234 - P273 - P280 - P305 + P351 + P338 - P390 - P391
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves