A5168

Adenosine Deaminase from bovine spleen

Type IX, ammonium sulfate suspension, 150-200 units/mg protein

• Synonym(s): Adenosine aminohydrolase
• CAS Number: 9026-93-1
• Enzyme Commission number: 3.5.4.4 (BRENDA, IUBMB)
• EC Number: 232-817-5
• MDL number: MFCD00081287
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bovine spleen
• Quality Level: 200
• type: Type IX
• form: ammonium sulfate suspension
• specific activity: 150-200 units/mg protein
• mol wt: 32.5-33 kDa
• UniProt accession no.: P56658
• foreign activity: 5′-AMP deaminase ≤0.01%; alkaline phosphatase ≤0.01%; guanase ≤0.01%; nucleoside phosphorylase ≤0.1%
• storage temp.: 2-8°C
• Gene Information: cow ... ADA(280712)

Description

General description

Adenosine deaminase/ADA is an enzyme, that is mainly present in lymphocytes and monocytes.

Molecular weight: 32.5-33 kDa

pI: 4.85

Application

Adenosine Deaminase from bovine spleen has been used to metabolize adenosine triphosphate (ATP) and adenosine to an inactive form (inosine). It has also been used to as an additive in KRH (Krebs−Ringer solution buffered with Hepes, containing CaCl2) medium in order to remove exogenous adenosine for cyclic adenosine monophosphate (cAMP) assay.

Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.

Biochem/physiol Actions

Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.

Adenosine deaminase/ADA can be used as an important marker to identify tuberculous pleural effusion. The activity of ADA is usually high in liver disease, tuberculosis, typhoid, infective mononucleosis and several malignancies.

Unit Definition

One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄, 0.01 M potassium phosphate, pH 6.0

Analysis Note

Protein determined by biuret.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves