38429

Laccase from Trametes versicolor

powder, light brown, ≥0.5 U/mg

• Synonym(s): Oxygen oxidoreductase
• CAS Number: 80498-15-3
• Enzyme Commission number: 1.10.3.2 (BRENDA, IUBMB)
• EC Number: 420-150-4
• MDL number: MFCD00131475
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: powder
• specific activity: ≥0.5 U/mg
• greener alternative product characteristics: Waste Prevention; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• color: light brown
• greener alternative category: Enabling,
• storage temp.: 2-8°C
• InChI: 1S/C9H13NO/c1-4-10-7(2)5-9(6-11)8(10)3/h5-6H,4H2,1-3H3
• InChI key: NWDZDFOKSUDVJV-UHFFFAOYSA-N

Description

General description

Laccase is an enzyme, produced by ericoid mycorrhiza and ectomycorrhiza fungi. It belongs to the group of polyphenol oxidases. Laccase is also present in plants and bacteria.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in fuel cell and cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.

Application

Laccase from Trametes versicolor has been used:

• to assess the use of four laccase-producing strains in waste water treatment
• in laccase assay
• in screening the lignols

Biochem/physiol Actions

Some of the enzymatic actions of laccase are associated with sporulation, detoxification, morphogenesis, melanin polymerization and it offers protection to spore coat. Laccase can catalyse a number of substrates including medicinal drugs and halogenated pesticides. It utilizes oxygen for its catalysis. For these reasons, it might be useful in the biological degradation of micropollutants in wastewater treatment.

Laccase catalyzes the oxidation of phenol containing compounds, including lignin, through the reduction of oxygen to water. The presence of mediators will allow the oxidation of non-phenlic compounds as well. The primary function of laccase is to degrade lignin in fungi.

Unit Definition

One unit corresponds to the amount of enzyme which converts 1 μmole of catechol per minute at pH 5.0 and 25 °C

Other Notes

former nomenclature: Coriolus versicolor

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P304 + P340 + P312 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves