G5882

Glutaraldehyde solution

Grade I, 25% in H₂O, specially purified for use as an electron microscopy fixative

• Synonym(s): Glutaric dialdehyde solution, Pentane-1,5-dial
• Linear Formula: OHC(CH₂)₃CHO
• CAS Number: 111-30-8
• Molecular Weight: 100.12
• Beilstein: 605390
• MDL number: MFCD00007025
• UNSPSC Code: 12352114
• PubChem Substance ID: 24895235
• NACRES: NA.21

Properties

• Quality Level: 300
• type: Grade I
• form: liquid
• concentration: 25% in H₂O
• technique(s): electron microscopy: suitable (fixative); electron microscopy: suitable
• color: colorless
• useful pH range: 2.9
• mp: -10 °C ((14 °F ))
• solubility: water: soluble at 20 °C
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: [H]C(CCCC([H])=O)=O
• InChI: 1S/C5H8O2/c6-4-2-1-3-5-7/h4-5H,1-3H2
• InChI key: SXRSQZLOMIGNAQ-UHFFFAOYSA-N

Description

General description

Glutaraldehyde (Glutaric dialdehyde, Pentane-1,5-dial) is an aliphatic dialdehyde widely utilized as a versatile biochemical reagent. Glutaraldehyde solutions contain both monomers and polymers with the difference in their fixation properties. Its applications include serving as a protein crosslinker, facilitating enzyme immobilization in microscopy, histochemistry, and cytochemistry. This compound acts as an amine-reactive homobifunctional crosslinker and a cell fixative before SDS-PAGE, staining, or electron microscopy, making it suitable for diverse morphological studies.
Glutaraldehyde efficiently crosslinks amine and hydrazine derivatives to proteins and other amine-containing polymers. Notably, it has been used in certain reactions to directly couple biotin hydrazides to nucleic acids, suggesting potential applications in conjugating fluorescent hydrazides and hydroxylamines to DNA. In histology studies, Glutaraldehyde is employed to preserve tissue sections, preparing them for closer examination.

Application

Glutaraldehyde solution has been used:

• to fix the blood samples for scanning electron microscopy (SEM)
• to fix scaffold pieces for SEM analysis
• in drug treatments to treat K562 (leukemia) cells to permanently cross-link cell components

Biochem/physiol Actions

Glutaraldehyde can be used in biomedical research to fix cells. It can also be used as a tool to investigate healthy and pathologic red blood cells. Medical equipment like spirometry tubing, endoscopes, dialyzers, hemodialysis proportioning and dialysate delivery systems, endocavitary probes, transducers, anesthesia and respiratory therapy equipment, and reuse of laparoscopic disposable plastic trocars can be disinfected with glutaraldehyde.

Features and Benefits

• Versatile and adaptable for wide variety of research applications
• Ready-made solution reduces the need for preparation time

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

For additional information on our range of Biochemicals, please complete this form.

Recommended for techniques demanding high purity.

To gain a comprehensive understanding of our extensive range of Monosaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Safety Information

• Pictograms: GHS08,GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H332,H314,H317,H334,H335,H410
• Precautionary Statements: P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves