72210

Neutral Red

for microscopy (Bact., Bot., Fl., Hist., Vit.), indicator (pH 6.8-8.0)

• Synonym(s): 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Basic Red 5, Toluylene red
• Empirical Formula (Hill Notation): C₁₅H₁₇ClN₄
• CAS Number: 553-24-2
• Molecular Weight: 288.78
• Colour Index Number: 50040
• Beilstein: 3918740
• EC Number: 209-035-8
• MDL number: MFCD00012651
• UNSPSC Code: 12171500
• PubChem Substance ID: 329763896
• NACRES: MA.02

Properties

• grade: for microscopy (Bact., Bot., Fl., Hist., Vit.); indicator (pH 6.8-8.0)
• Quality Level: 100
• form: powder or crystals
• technique(s): titration: suitable
• pH: 6.8-8.0, red to yellow
• mp: 290 °C (dec.) (lit.)
• solubility: water: 0.01 g/10 mL
• εmax: ≥800 at 535-545 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1
• InChI: 1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H
• InChI key: PGSADBUBUOPOJS-UHFFFAOYSA-N

Description

General description

Neutral red is a vital dye widely used as a cellular pH indicator and is selective for shifts in pH. It is highly lipophilic in nature. It favours membrane potential dependent fluorescence that attributes to changes in hydrophobicity.

Application

Neutral red has been used to stain mouse mammary glands.

Useful as an indicator for preparing neutral red paper, and as a biological stain.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves