42579
4-Phenyl-1,2,4-triazoline-3,5-dione
for HPLC derivatization, LiChropur™, ≥98.0% (CHN)
Synonym(s):
Cookson reagent, 4-Phenyl-3H-1,2,4-triazole-3,5(4H)-dione, PTAD
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About This Item
Empirical Formula (Hill Notation):
C8H5N3O2
CAS Number:
Molecular Weight:
175.14
Beilstein:
141548
EC Number:
MDL number:
UNSPSC Code:
41116105
PubChem Substance ID:
NACRES:
NA.22
Recommended Products
grade
for HPLC derivatization
Quality Level
Assay
≥98.0% (CHN)
quality
LiChropur™
technique(s)
HPLC: suitable
mp
165-170 °C (dec.) (lit.)
storage temp.
2-8°C
SMILES string
O=C1N=NC(=O)N1c2ccccc2
InChI
1S/C8H5N3O2/c12-7-9-10-8(13)11(7)6-4-2-1-3-5-6/h1-5H
InChI key
ISULLEUFOQSBGY-UHFFFAOYSA-N
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General description
4-Phenyl-1,2,4-triazoline-3,5-dione, also known as Cookson reagent, is a strong dienophile, which gives a stable Diels-Alder adduct quantitatively within a short time and under mild conditions. It is commonly used as a protecting group of the diene moiety for the synthesis of vitamin D3 (VD3)-related compounds.
Application
4-Phenyl-1,2,4-triazoline-3,5-dione may be used as a derivatizing reagent for the determination of trace levels of 25-hydroxyvitamin D and its C-3 epimer in biological samples and cholecalciferol (vitamin D3) in fortified infant formula, milk and milk powder using liquid chromatography–tandem mass spectrometry (LC–MS/MS)technique.
Legal Information
LiChropur is a trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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