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Key Documents

38978

Sigma-Aldrich

Malachite Green solution

for microscopy

Synonym(s):

Basic Green 4, China Green, Victoria Green B, Aniline Green, Benzaldehyde green, CI 42000

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
MA.02

grade

for microscopy

Quality Level

product line

BioChemika

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

suitability

bacteria

SMILES string

OC(=O)C([O-])=O.CN(C)c1ccc(cc1)/C(c2ccccc2)=C3/C=C\C(C=C3)=[N+](/C)C

InChI

1S/C23H25N2.ClH/c1-24(2)21-14-10-19(11-15-21)23(18-8-6-5-7-9-18)20-12-16-22(17-13-20)25(3)4;/h5-17H,1-4H3;1H/q+1;/p-1

InChI key

FDZZZRQASAIRJF-UHFFFAOYSA-M

General description

Malachite Green is used for bacterial spore staining by Schaeffer and Fulton`s method. It can also be used in place of methyl green in the Pappenheim stain, when combined with the Gram stain.

Application

Staining of bacterial spores according to Schaeffer and Fulton; Pappenheim stain when combined with Gram′s stain

Components

Composition
Malachite green 10 g/l, in water (oxalate form is used)

replaced by

Hazard Statements

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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R Sahraei et al.
Environmental monitoring and assessment, 185(7), 5817-5822 (2012-12-04)
A new spectrophotometric method is reported for the determination of nanomolar level of malachite green in surface water samples. The method is based on the catalytic effect of silver nanoparticles on the oxidation of malachite green by hexacyanoferrate (III) in
P Manoj Kumar Reddy et al.
Water science and technology : a journal of the International Association on Water Pollution Research, 67(5), 1097-1104 (2013-02-19)
Oxidative decomposition of aqueous organic pollutant malachite green (MG) was studied in a dielectric barrier discharge reactor operated under ambient conditions. Total organic carbon content analysis confirmed the mineralization of the pollutant leading to the formation of carbon dioxide, which
Ziru Lian et al.
Marine pollution bulletin, 64(12), 2656-2662 (2012-11-06)
In this paper, a highly selective sample cleanup procedure combining molecular imprinting technique (MIT) and solid-phase extraction (SPE) was developed for the isolation of malachite green in seawater and seafood samples. The molecularly imprinted polymer (MIP) was prepared using malachite
Jia Zeng et al.
Biophysical journal, 104(1), 139-145 (2013-01-22)
It is shown that the nonlinear optical phenomenon known as second-harmonic generation can be used for label-free, time-resolved study of the transport of molecules through living cell membranes. The adsorption and transport of a 300-Da molecular-mass hydrophobic ion at the
Randall Reif et al.
Nucleic acid therapeutics, 22(6), 428-437 (2012-11-02)
Due to the discovery of more and more roles of cellular noncoding RNAs, the approaches for introducing RNAs including small interfering RNA (siRNA), micro RNA (miRNA), ribozyme, and riboswitch into cells for regulating cell life cycle and for the treatment

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