17779
2,3,5-Triphenyl-tetrazolium chloride solution
suitable for microbiology, Filter sterilized solution that is recommended for the detection of microbial growth based on reduction of TTC
Synonym(s):
TTC solution, TTC supplement, TTC solution, TTC supplement
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About This Item
Empirical Formula (Hill Notation):
C19H15ClN4
CAS Number:
Molecular Weight:
334.80
Beilstein:
3923356
MDL number:
UNSPSC Code:
41171614
PubChem Substance ID:
NACRES:
NA.85
Recommended Products
Quality Level
sterility
sterile (Filtered and Aseptic Handled)
form
liquid
shelf life
limited shelf life, expiry date on the label
mol wt
334.8 g/mol
application(s)
agriculture
environmental
food and beverages
microbiology
storage temp.
2-8°C
suitability
bacteria
SMILES string
[Cl-].c1ccc(cc1)-c2nn(-c3ccccc3)[n+](n2)-c4ccccc4
InChI
1S/C19H15N4.ClH/c1-4-10-16(11-5-1)19-20-22(17-12-6-2-7-13-17)23(21-19)18-14-8-3-9-15-18;/h1-15H;1H/q+1;/p-1
InChI key
PKDBCJSWQUOKDO-UHFFFAOYSA-M
General description
2,3,5-Triphenyl-tetrazolium chloride (TTC) is a dye largely used for the easy enumeration of microbial colonies in solid culture media. This dye is colorless in the oxidized form and changes to red when reduced by microorganisms, due to the formation of formazan. Live microorganisms, for example, E. coli and other coliforms reduce TTC by enzymatic action, forming red color formazan that is kept inside granules in the cells. TTC is highly recommended in testing pasteurized milk because there is a high percentage of microorganisms unable to reduce TTC in pasteurized milk, which cannot be detected by laboratory procedures. Higher concentrations of TTC may have an inhibitory effect on the growth of Gram-positive organisms.
Application
2,3,5-Triphenyl-tetrazolium chloride (TTC) solution is a redox indicator that can help indicate cellular respiration and identify viable and non-viable cells as well as identify microorganisms capable of reduction of TTC on solid culture media
Components
(per vial)
2,3,5-Triphenyl tetrazolium chloride: 0.1 g, distilled water: 10 ml
2,3,5-Triphenyl tetrazolium chloride: 0.1 g, distilled water: 10 ml
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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