MABC942
Anti-PLA2R Antibody, clone 5F5.1
clone 5F5.1, from mouse
Synonym(s):
PLA2-R, PLA2R, Secretory phospholipase A2 receptor, 180 kDa secretory phospholipase A2 receptor, C-type lectin domain family 13 member C, M-type receptor
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
5F5.1, monoclonal
species reactivity
human
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PLA2R1(22925)
General description
Secretory phospholipase A2 receptor (UniProt Q13018; also known as 180 kDa secretory phospholipase A2 receptor, C-type lectin domain family 13 member C, M-type receptor, Phospholipase A2 receptor 1) is encoded by the PLA2R1 (also known as CLEC13C, PLA2G1R, PLA2R, PLA2IR) gene (Gene ID 22925) in human. PLA2R is initially produced with a signal peptide sequence (a.a. 1-20), the cleavage of which yields the mature type I transmembrane receptor composed of a short cytoplasmic tail (a.a. 1419-1463), a transmembrane segment (a.a. 1419-1463), and a large extracellular portion consisting of an N-terminal cystein-rich region, a fibronectin-like type II domain, and a tandem repeat of eight carbohydrate-recognition domains (CRDs). Both group IB and X secretory phospholipase A2 (sPLA2) enzymes are identified as ligands orf PLA2R, while group IIA sPLA2s bind PLA2R with about 10-fold lower affinity when compared with group IB and X sPLA2s. In mice, the extracellular portion of PLA2R is reported to be cleaved off into a soluble form (sPLA2R) in blood, where it functions as a circulating sPLA2 inhibitory protein (PLI) that blocks both the receptor-binding and enzymatic activity of sPLA2.
Specificity
Expected to react with both spliced isoforms, as well as the soluble receptor (sPLA2R) derived from metalloproteinases-mediated cleavage of the membrane-bound receptor.
Immunogen
Epitope: Extracellular C-type lectin domains 3-5
GST-tagged recombinant protein corresponding to the extracellular C-type lectin domains 3-5 of human PLA2R.
Application
This Anti-PLA2R Antibody, clone 5F5.1 is validated for use in Western Blotting and Immunohistochemistry for the detection of PLA2R.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected PLA2R in 10 µg of human kidney tissue lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected PLA2R in human kidney tissue.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected PLA2R in human kidney tissue.
Quality
Evaluated by Western Blotting in human lung tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PLA2R in 10 µg of human lung tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PLA2R in 10 µg of human lung tissue lysate.
Target description
~180 kDa observed. Target band(s) appears larger than the calculated molecular weights (166.6 kDa/isoform 1 and 150.8 kDa/isoform 2) due to posttranslational glycosylation. Uncharacterized band(s) may appear in some lysates.
Physical form
Format: Purified
Other Notes
Concentration: Please refer to lot specific datasheet.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Astgik Petrosyan et al.
Nature communications, 10(1), 3656-3656 (2019-08-15)
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The deposition of antipodocyte autoantibodies in the glomerular subepithelial space induces primary membranous nephropathy (MN), the leading cause of nephrotic syndrome worldwide. Taking advantage of the glomerulus-on-a-chip system, we modeled human primary MN induced by anti-PLA2R antibodies. Here we show
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