861049

Azure A chloride

certified by the Biological Stain Commission, Dye content 70 %

• Synonym(s): Methylene blue
• Empirical Formula (Hill Notation): C₁₄H₁₄ClN₃S
• CAS Number: 531-53-3
• Molecular Weight: 291.80
• Colour Index Number: 52005
• Beilstein: 3922287
• EC Number: 208-510-7
• MDL number: MFCD00012112
• UNSPSC Code: 12171500
• PubChem Substance ID: 329769437
• NACRES: NA.47

Properties

• grade: certified by the Biological Stain Commission
• Quality Level: 200
• form: powder
• composition: Dye content, 70%
• color: blue to very dark blue; very dark green
• mp: 290 °C (dec.) (lit.)
• solubility: H₂O: 1 mg/mL
• λmax: 633 nm
• ε (extinction coefficient): ≥11000 at 242-246 nm in H₂O at -1; ≥26000 at 287-291 nm in H₂O; ≥34000 at 620-634 μm in H₂O
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: NC1=CC(SC(C2=N3)=CC(C=C2)=[N+](C)C)=C3C=C1.[Cl-]
• InChI: 1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
• InChI key: NALREUIWICQLPS-UHFFFAOYSA-N

Description

Application

Azure A has been used to determine the amount of charged dextran sulfate in the nanoparticles for the study involving incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles.

Biochem/physiol Actions

The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. Azure A is a main component of the azure B/polychromed methylene blue-eosin stains family, also referred to as the Romanowsky stain. Azure A is a basic cationic dye.

Suitability

Certified for use in Lillie′s modified Nocht′s method and the NCCLS method for blood smears.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves