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T7024

Sigma-Aldrich

N,N,N′,N′-Tetramethylethylenediamine

BioReagent, for molecular biology, ≥99% (GC)

Synonym(s):

1,2-Bis(dimethylamino)ethane, TEMED, TMEDA

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About This Item

Linear Formula:
(CH3)2NCH2CH2N(CH3)2
CAS Number:
110-18-9
Molecular Weight:
116.20
Beilstein:
1732991
EC Number:
203-744-6
MDL number:
MFCD00008335
UNSPSC Code:
41105319
PubChem Substance ID:
24900242
NACRES:
NA.31

grade

for molecular biology

product line

BioReagent

Assay

≥99% (GC)

expl. lim.

9.08 %

refractive index

n20/D 1.4179 (lit.)

bp

120-122 °C (lit.)

mp

−55 °C (lit.)

density

0.775 g/mL at 20 °C (lit.)

foreign activity

DNase, RNase, protease, none detected

SMILES string

CN(C)CCN(C)C

InChI

1S/C6H16N2/c1-7(2)5-6-8(3)4/h5-6H2,1-4H3

InChI key

KWYHDKDOAIKMQN-UHFFFAOYSA-N

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Application

This product is designated as molecular biology grade and is suitable for polyacrylamide gel electrophoresis (PAGE). It has been analyzed for the absence of nucleases and proteases.
TEMED is a tertiary amine that is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. TEMED-generated free radicals cause the compounds acrylamide and bis-acrylamide to polymerize and form the gel matrix polyacrylamide. Because TEMED is functional only in its free base form, low pH conditions will inhibit polymerization.,,
The potential contribution of TEMED to elevated background in silver stained SDS PAGE has been studied. TEMED has been used in the coating of soft polymers, such as polyacrylamide, in capillaries for investigation by atomic force microscopy. Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED.
The capillary isoelectric focusing of proteins with an isoelectric point (pI) > 12 has been investigated through the use of TEMED. TEMED has been used in the HPLC of small organic molecules.

Quantity

Suggested use is 35 μl for 50 ml acrylamide gel solution.

Signal Word

Danger

Hazard Statements

H225,H302,H314,H331

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

61.7 °F - closed cup

Flash Point(C)

16.5 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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H Rosing et al.
Journal of chromatography. B, Biomedical applications, 668(1), 107-115 (1995-06-09)
A sensitive high-performance liquid chromatographic (HPLC) assay has been developed and validated for the quantitation of the novel anticancer agent topotecan and topotecan as the total of its lactone and carboxylate forms in human plasma. Linear response in analyte standard
G Patras et al.
Electrophoresis, 20(10), 2039-2045 (1999-08-18)
A novel experimental procedure to study the factors that contribute to background silver staining in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is described. A mechanism is proposed for background silver staining, which was found to result primarily from the redox
Andrews, A. T.
Electrophoresis: Theory, Techniques, and Biochemical and Clinical Applications, 21-24 (1986)
Sambrook, J. and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 5-5 (2001)
A V Vasiliskov et al.
BioTechniques, 27(3), 592-594 (1999-09-18)
The manufacturing of microchips containing oligonucleotides and proteins immobilized within gel pads, ranging in size from 10 x 10 to 100 x 100 microns, is described. The microchips are produced by photo- or persulfate-induced copolymerization of unsaturated derivatives of biomolecules
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