SRP0166

DNMT3B/DNMT3L Active human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

• Synonym(s): DNA (cytosine-5-)-methyltransferase 3b, ICF, M.HsaIIIB
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• Assay: ≥90% (SDS-PAGE)
• form: aqueous solution
• mol wt: 27 kDa (DNMT3L); 59 kDa (DNMT3B)
• packaging: pkg of 10 μg
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_006892 (DNMT3B); NM_013369 (DNMT3L)
• UniProt accession no.: Q9UBC3
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... DNMT3B(1789)

Description

General description

DNA (cytosine-5-)-methyltransferase 3 β (DNMT3B) has two subfamilies-DNMT3B and ΔDNMT3B. These are expressed in many transcript variants. Several single-nucleotide polymorphisms are observed within the promoter region of the DNMT3B gene. The gene encoding this protein is localized on human chromosome 20q11.2. DNA (cytosine-5)-methyltransferase 3-like (DNMT3L) is part of the DNA methyltransferases family. It has a cysteine-rich region containing a novel-type zinc finger domain. The gene encoding it is localized on human chromosome 21q22.3.

Application

Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

DNA (cytosine-5-)-methyltransferase 3 β (DNMT3B) is a de novo methyltransferase which targets the un-methylated 5′-C-phosphate-G-3′ (CpG) sites on the gene promoter. It is responsible for crucial epigenetic mechanisms for the regulation of chromosomal stability and for building a new methylation pattern. The protein is associated with promoter hypermethylation in tumorigenesis and lung cancer. DNA (cytosine-5)-methyltransferase 3-like (DNMT3L) is a non-catalytic accessory factor which is mainly responsible for the stimulation of DNA methylation. It helps the DNA methylation machinery to be positioned on properly chromatinized DNA regions. The protein also helps to provide uniform methylation patterns. It has an important role in spermatogenesis and mutations in the DNMT3L gene might be associated with azoospermia susceptibility.

Physical form

Formulated in: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCL, 20% glycerol, 4 mM glutathione.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable