G1794

GAP-27

≥97% (HPLC)

• Synonym(s): Ser-Arg-Pro-Thr-Glu-Lys-Thr-Ile-Phe-Ile-Ile
• Empirical Formula (Hill Notation): C₆₀H₁₀₂N₁₅O₁₇
• CAS Number: 198284-64-9
• Molecular Weight: 1305.54
• MDL number: MFCD03456920
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 200
• Assay: ≥97% (HPLC)
• form: powder
• packaging: pkg of 2 mg
• technique(s): cell culture | mammalian: suitable
• solubility: water: soluble 1.00-1.02 mg/mL, clear, colorless
• UniProt accession no.: P35212
• shipped in: ambient
• storage temp.: −20°C
• InChI: 1S/C60H101N15O17/c1-9-31(4)44(54(86)69-41(29-36-19-13-12-14-20-36)52(84)70-45(32(5)10-2)55(87)72-46(59(91)92)33(6)11-3)71-57(89)48(35(8)78)73-51(83)38(21-15-16-26-61)66-50(82)39(24-25-43(79)80)67-56(88)47(34(7)77)74-53(85)42-23-18-28-75(42)58(90)40(22-17-27-65-60(63)64)68-49(81)37(62)30-76/h12-14,19-20,31-35,37-42,44-48,76-78H,9-11,15-18,21-30,61-62H2,1-8H3,(H,66,82)(H,67,88)(H,68,81)(H,69,86)(H,70,84)(H,71,89)(H,72,87)(H,73,83)(H,74,85)(H,79,80)(H,91,92)(H4,63,64,65)/t31-,32-,33-,34+,35+,37-,38-,39-,40-,41-,42-,44-,45-,46-,47-,48-/m0/s1
• InChI key: SXRAPDIXXYFGJG-MDAHIHQXSA-N
• Gene Information: human ... GJA4(2701)

Description

Amino Acid Sequence

Ser-Arg-Pro-Thr-Glu-Lys-Thr-Ile-Phe-Ile-Ile

Application

GAP-27 has been used as a blocking peptide for Cx43 (connexin 43) gap junctions in FDC (follicular dendritic cells)-B cell cultures.

Biochem/physiol Actions

GAP-27 shares homology with a region of extracellular loops of connexins, and hence, is thought to inhibit junctions formed by the connexins Cx32, Cx40 and Cx43. It highly hinders co-operative cell-cell interactions including the synergized beating of embryonic cardiomyocytes. This peptide reduces osteoclastic activity and survival rates. This peptide significantly reduces acetylcholine-mediated hyperpolarization of smooth muscle cells.

Inhibitor of gap junction signaling in endothelial cells.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves