D1313
JumpStart™ REDAccuTaq® LA DNA Polymerase
Long and accurate hot-start Taq with inert dye, 10X buffer included
Synonym(s):
High fidelity Taq, Hot start DNA polymerase, Hot start Taq, Long Taq
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About This Item
UNSPSC Code:
41106314
NACRES:
NA.55
Recommended Products
form
liquid
usage
sufficient for 250 reactions
sufficient for 50 reactions
feature
Long & Accurate PCR
dNTPs included: no
hotstart
concentration
1 unit/μL
technique(s)
PCR: suitable
color
red
input
purified DNA
suitability
suitable for PCR
application(s)
agriculture
shipped in
wet ice
storage temp.
−20°C
General description
JumpStart™ REDAccuTaq® LA DNA Polymerase contains AccuTaq long and accurate (LA) DNA polymerase, an inert red dye, and JumpStart Taq antibody. This enzyme is suitable for long-distance and high-fidelity PCR, multiplex PCR, and PCR amplification of targets with variable lengths, such as amplification of cDNA libraries. It enables the amplification from 0.25 to 22 kb for complex genomic DNA and up to 40 kb for less complex templates. JumpStart Taq antibody is designed to minimize non-specific amplification while increasing target yield. Unlike other Hot-start methods (i.e. chemical inactivation), the JumpStart Taq antibody does not require a pre-incubation step before cycling because polymerase activity is fully restored during the first denaturation cycle of the PCR reaction. The PCR product can be easily separated from the red dye by standard purification methods. The inert red dye has does not affect automated sequencing, restriction enzyme digestion, ligation, or other downstream applications. Its high fidelity makes it the enzyme of choice when performing amplifications where a low error frequency is critical, such as in RT-PCR and cloning.
Application
JumpStart™ REDAccuTaq® LA DNA Polymerase has been used for amplifying genomic DNA. It has also been used in polymerase chain reaction (PCR) for gene cloning.
JumpStart REDAccuTaq LA DNA Polymerase is a unique enzyme blend that is capable of generating long PCR fragments, from 0.25 kb to 40 kb, with high fidelity, increased specificity and yield. JumpStart REDAccuTaq DNA polymerase combines Sigma′s AccuTaq LA DNA polymerase and JumpStart Taq antibody with an inert red dye. This specially formulated hot start enzyme mix achieves greater yields, enhances sensitivity and results in higher fidelity (6.5×) in comparison to standard Taq or other Long and Accurate enzyme blends. Its high fidelity makes it the enzyme of choice when performing amplifications where a low error frequency is critical, such as in RT-PCR and cloning.
JumpStart REDAccuTaq LA DNA Polymerase also contains the hot start mechanism of the JumpStart Taq antibody. JumpStart Taq antibody is designed to minimize non-specific amplification while increasing target yield. Unlike other hot-start methods (i.e. chemical inactivation), JumpStart Taq antibody does not require a pre-incubation step prior to cycling because polymerase activity is fully restored during the first denaturation cycle of the PCR reaction.
The inert red dye provides quick recognition and confirmation of appropriate mixing. An aliquot of the samples (5-10 μL) may be loaded directly onto an agarose gel following PCR. The red dye migrates slightly faster than bromophenol blue at the same rate as a 125 base pair fragment.
The PCR product can be easily separated from the dye by standard purification methods. The inert red dye has does not effect automated sequencing, restriction enzyme digestion, ligation or other downstream applications.
JumpStart REDAccuTaq LA DNA Polymerase also contains the hot start mechanism of the JumpStart Taq antibody. JumpStart Taq antibody is designed to minimize non-specific amplification while increasing target yield. Unlike other hot-start methods (i.e. chemical inactivation), JumpStart Taq antibody does not require a pre-incubation step prior to cycling because polymerase activity is fully restored during the first denaturation cycle of the PCR reaction.
The inert red dye provides quick recognition and confirmation of appropriate mixing. An aliquot of the samples (5-10 μL) may be loaded directly onto an agarose gel following PCR. The red dye migrates slightly faster than bromophenol blue at the same rate as a 125 base pair fragment.
The PCR product can be easily separated from the dye by standard purification methods. The inert red dye has does not effect automated sequencing, restriction enzyme digestion, ligation or other downstream applications.
Features and Benefits
- JumpStart REDAccuTaq LA DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity
- Up to 6.5X greater fidelity in comparison to Taq DNA polymerase making it the ideal enzyme for multiplex PCR
- Produce amplicons up to 22 kb with genomic templates and up to 40 kb with less complex templates such as lambda or bacterial genomic DNA
- Reduce set-up time and eliminate concerns associated with manual or wax Hot Start methods
- Dye allows for quick visual confirmation that reagent has been added and mixed properly
- Direct loading onto an agarose gel without additional dyes
Packaging
Supplied with optimized 10× reaction buffer
Unit Definition
One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min at 74 °C.
Other Notes
View more detailed information on JumpStart REDTaq and Accutaq enzymes at www.sigma-aldrich.com/hotstart.
Legal Information
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDAccuTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
related product
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Long and accurate PCR applications address the needs for longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase.
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
Protocols
Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
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