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C8180

Sigma-Aldrich

Cholera Toxin A Subunit from Vibrio cholerae

lyophilized powder

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About This Item

MDL number:
UNSPSC Code:
12352200
NACRES:
NA.77

form

lyophilized powder

Quality Level

composition

Protein, ~5% Lowry

availability

not available in Japan

impurities

≤0.5% Cholera toxin B subunit (SDS-PAGE)

storage temp.

2-8°C

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General description

Cholera Toxin (CT) is secreted by Vibrio cholerae. CT upregulates cell surface molecules, such as co-stimulatory molecules and chemokine receptors in antigen presenting cells(APCs), murine and human dendritic cells (DCs). It enhances the secretion of interleukin 1 (IL-1) from macrophages and stimulates their APC function. CT also functions as an immunomodulator.

Application

Cholera Toxin A Subunit from Vibrio cholerae has been used as a specific probe for trimeric G proteins in membrane motility assays.

Biochem/physiol Actions

Catalyzes ADP-ribosylation of the α-subunit of G proteins, reducing GTPase activity and activating the α-subunit; also catalyzes ADP-ribosylation of cell membrane adenylyl cyclase

Physical form

Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide and sodium EDTA

Reconstitution

When reconstituted with 1 mL of water, the solution will contain 0.05 M Tris buffer salts, pH 7.5, 0.2 M NaCl, 0.003 M NaN3, and 0.001 M sodium EDTA. Store reconstituted solutions in the refrigerator.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Inhalation - Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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In Vitro Reconstitution of Microtubule Plus End-directed, GTPgammaS-sensitive Motility of Golgi Membranes
Fullerton AT, et al.
Molecular Biology of the Cell, 9(10), 2699-2714 (1998)
Actions of cholera toxin and the prevention and treatment of cholera
Holmgren J.
Nature, 292(5822), 413-413 (1981)
Cholera toxin: a paradigm of a multifunctional protein
Bharati K and Ganguly NK
The Indian Journal of Medical Research, 133(2), 179-179 (2011)
Irina N Marinova et al.
STAR protocols, 2(3), 100668-100668 (2021-09-07)
Glycosylation is one of the most common protein modifications in living organisms and has important regulatory roles in animal tissue development and homeostasis. Here, we present a protocol for generation of 3D organotypic skin models using CRISPR-Cas9 genetically engineered human
Jiwei Liu et al.
EMBO reports, 21(2), e48597-e48597 (2019-12-14)
Histone lysine demethylase 1 (LSD1), the first identified histone demethylase, is overexpressed in multiple tumor types, including breast cancer. However, the mechanisms that cause LSD1 dysregulation in breast cancer remain largely unclear. Here, we report that protein arginine methyltransferase 4

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