C4187
Cyanoborohydride Coupling Buffer
Synonym(s):
Coupling buffer
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About This Item
UNSPSC Code:
12161700
NACRES:
NA.56
Recommended Products
form
liquid
reaction suitability
reagent type: reductant
technique(s)
affinity chromatography: suitable
application(s)
life science and biopharma
storage temp.
2-8°C
Application
A ready-to-use reagent used to couple amine ligands to aldehyde functional groups. The coupling buffer reaction is a reductive amination of the intermediate Schiff′s base to a stable C−N bond.
Cyanoborohydride Coupling Buffer has been used:
- in coupling reactions between amines and glutaraldehyde
- to reduce hydrazone bond to a stable hydrazide bond
- as a component in oligonucleotide reaction mixture for coverslips functionalization
Cyanoborohydride Coupling Buffer is used in affinity chromatography, protein chromatography, activated/functionalized matrices and synthetic reagents. Cyanoborohydride has been used to inform a safe and effective gene-transfer system targeting hepatocytes as well as to develop a method for targeted delivery of anticancer therapeutics to cancer cells in hypoxic areas.
Biochem/physiol Actions
Cyanoborohydride Coupling Buffer is a reagent suitable for reductive amination processes, that contributes to transformation of simple alcohols into more complex amines. It is used in the conversion of Schiff base, by reducing it, to form a secondary amine without affecting aldehyde groups on the support.
Components
0.02 M sodium phosphate, pH 7.5, containing 0.2 M sodium chloride and 3.0 g/L sodium cyanoborohydride
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Binding between the integrin ?X?2 (CD11c/CD18) and heparin.
Vorup-Jensen T, et al.
Test, 282(42), 30869-30877 (2007)
Christopher A Holden et al.
International journal of nanomedicine, 5, 25-36 (2010-02-18)
Tumors frequently contain hypoxic regions that result from a shortage of oxygen due to poorly organized tumor vasculature. Cancer cells in these areas are resistant to radiation- and chemotherapy, limiting the treatment efficacy. Macrophages have inherent hypoxia-targeting ability and hold
Use of polymer supported reagents for clean multi-step organic synthesis: preparation of amines and amine derivatives from alcohols for use in compound library generation
Ley S, et al.
Journal of the Chemical Society. Perkin Transactions 1, 15(27), 2239-2242 (1998)
Handbook of Affinity Chromatography (2005)
V S Hornsey et al.
Journal of immunological methods, 93(1), 83-88 (1986-10-23)
For coupling proteins to Sephacryl gels periodate oxidation of these gels was investigated as an alternative method to cyanogen bromide activation. Optimum conditions were studied with respect to periodate concentration, time of oxidation, pH and type of coupling buffer, concentration
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