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C3865

Sigma-Aldrich

Anti-N-Cadherin antibody, Mouse monoclonal

clone GC-4, purified from hybridoma cell culture

Synonym(s):

Anti-A-CAM, Anti-A-Cell Adhesion Molecule

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About This Item

MDL number:
MFCD00164511
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

GC-4, monoclonal

form

buffered aqueous solution

mol wt

antigen 135 kDa

species reactivity

chicken, monkey, mouse, human, rabbit, rat

technique(s)

electron microscopy: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: 10-20 μg/mL using rat cardiac muscle frozen sections
western blot: 10-20 μg/mL using chicken cardiac muscle or COS-7 cell extracts

isotype

IgG1

UniProt accession no.

P19022

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CDH2(1000)
mouse ... Cdh2(12558)
rat ... Cdh2(83501)

General description

N-Cadherin is a member of the cadherin family of proteins that mediate cell-cell adhesion in a calcium dependent manner. N-cadherin is a transmembrane glycoprotein that localizes in the cell-cell adherens junction on the plasma membrane. The protein is known to be localized in the cell membrane.

Application

Anti-N-Cadherin antibody is suitable for the following procedures:
  • electron microscopy
  • flow cytometry
  • immunocytochemistry
  • immunohistochemistry at a concentration of 10-20μg/mL using rat cardiac muscle frozen sections
  • western blot at a concentration of 10-20μg/mL using chicken cardiac muscle or COS-7 cell extracts

Biochem/physiol Actions

CDH11 gene encodes a type II classical cadherin that mediate calcium-dependent cell-cell adhesion.
Higher expression of CDH2 gene was observed in tumor cells. It is known to induce the invasive and chemoresistant attributes to the cancer cells. N-glycosylation of N-cadherin is associated with the process of tumorigenesis especially in mammary gland, renal system, and ovary. Loss of expression of the gene was observed in adrenocortical carcinoma.

Physical form

0.2 μm-filtered solution in 0.01 M phosphate buffered saline, pH 7.4.

Other Notes

Clone GC-4 is also known as GB-9.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Priscilla Y Hwang et al.
Cellular and molecular bioengineering, 8(1), 51-62 (2015-04-08)
Juvenile nucleus pulposus (NP) cells of the intervertebral disc (IVD) are large, vacuolated cells that form cell clusters with strong cell-cell interactions. With maturation and aging, NP cells lose their ability to form these cell clusters, with aging-associated changes in
Priscilla Y Hwang et al.
Scientific reports, 6, 28038-28038 (2016-06-14)
Nucleus pulposus (NP) cells of the intervertebral disc are essential for synthesizing extracellular matrix that contributes to disc health and mechanical function. NP cells have a unique morphology and molecular expression pattern derived from their notochordal origin, and reside in
Rongbin Ge et al.
Oncotarget, 6(30), 28973-28987 (2015-09-12)
Metformin has emerged as a potential anticancer agent. Here, we demonstrate that metformin plays an anti-tumor role via repressing N-cadherin, independent of AMPK, in wild-type N-cadherin cancer cells. Ectopic-expression of N-cadherin develops metformin-resistant cancer cells, while suppression of N-cadherin sensitizes
Marcin P Iwanicki et al.
JCI insight, 1(10) (2016-08-03)
High-grade serous ovarian carcinoma (HGS-OvCa) harbors p53 mutations and can originate from the epithelial cell compartment of the fallopian tube fimbriae. From this site, neoplastic cells detach, survive in the peritoneal cavity, and form cellular clusters that intercalate into the
Meng-Hsuan Wen et al.
Traffic (Copenhagen, Denmark), 17(7), 769-785 (2016-04-12)
Macropinocytosis is a clathrin-independent endocytic pathway implicated in fluid uptake, pathogen invasion and cell migration. During collective cell migration, macropinocytosis occurs primarily at membrane ruffles arising from the leading edges of migrating cells. We report here that N-cadherin (Ncad) regulates
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