APPA014

HIV Protease Activity Detection Kit

• Synonym(s): Cut-N-Glow HIV Protease Detection Kit
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• form: solution
• Quality Level: 200
• shipped in: dry ice
• storage temp.: −20°C

Description

Application

This kit is specific for the HIV Protease, a retroviral aspartyl protease that is critical to viral life-cycle. The Cut-N-Glow approach involves the introduction of a structural distortion into one of the complementary fragments (GFP 11), through the use of a conditionally stable tether, which serves to constrain the N and C termini of GFP 11 closely in space, thereby, diminishing the mutual affinity of the two fragments and blocking protein self-assembly until the tether is cleaved. The distortion can be reversed upon site-specific proteolysis of the tether, resulting in GFP 11 fragment assembly with GFP 1-10, generating reconstituted, functional GFP. Depending on the tether, the chimeric GFP can serve as a substrate for proteases from the three major classes: serine, cysteine, and aspartic acid.

Suitability

This kit contains sufficient reagents for one 96 well plate (96 tests)

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable