Skip to Content
Merck
All Photos(1)

Documents

A4955

Sigma-Aldrich

Alkaline Phosphatase Stabilizing Buffer

liquid

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

sterility

non-sterile

Quality Level

form

liquid

technique(s)

ELISA: suitable

storage temp.

2-8°C

General description

Alkaline Phosphatase Stabilizing Buffer can extend the shelf life of alkaline phosphate conjugates.

Application

Alkaline Phosphatase (ALP) Stabilizing Buffer may be used in ALP assay in osteoblast cell protein lysates. It may also be used as a component of antibody-enzyme conjugate dialysis buffer.
Alkaline Phosphatase Stabilizing Buffer has been tested in ELISA systems. The product has been specially made for the stable dilution of alkaline phosphatase-antibody conjugates.It can be used for a series of experiments to achieve consistent results.

Components

Stabilizing compounds in Tris buffered saline, pH 8.0, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

The degree of stability realized is highly dependent upon the conjugate tested. Trials using each particular conjugate should be performed to determine the degree of stability that can be expected. It is recommended that this buffer constitute ≥95% of the final solution.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Development of biosensor array for rapid detection of cardiac markers: Immunosensor for detection of free cardiac troponin I
ORegan T, et al.
Analytical Letters, 36(9), 1903-1920 (2003)
Shi Bai et al.
Scientific reports, 8(1), 1048-1048 (2018-01-20)
Guanylate Binding Proteins (GBPs) are a group of cytokine-inducible large guanosine triphosphatase. Previous studies have shown high expression of GBP1 in circulating monocytes of premenopausal subjects was correlated to extremely low peak bone mass, which is considered as an important
Xuefeng Zhao et al.
Scientific reports, 6, 19296-19296 (2016-01-15)
Mesenchymal stem cells (MSCs) are multipotent cells, which can give rise to variety of cell types, including adipocytes and osteoblasts. Previously, we have shown that cysteine dioxygenase type 1 (Cdo1) promoted adipogenesis of primary mouse bone marrow stromal cells (BMSCs)
Tanu Sharma et al.
Nutrition and cancer, 72(5), 873-883 (2019-08-15)
Microcalcification seems to be an assurance signature for the prediction of breast cancer malignancy. However, neither systematic study for deciphering the molecular mechanism of mammary microcalcification has yet been conducted, nor a mechanistic study has been performed to find out

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service