MTOX1000CC

Caco-2 Engineered Control Cells

Human male colorectal tissue, adenocarcinoma

• Synonym(s): Caco-2 cell line
• UNSPSC Code: 41106514

Properties

• product name: Caco-2 Engineered Control Cells, one vial of frozen cells
• biological source: human male colorectal tissue (Source Disease: adenocarcinoma)
• form: liquid
• storage temp.: −196°C

Description

General description

The C2BBe1 cells, a sublcone of Caco-2 cells, correspond to ATCC CRL-2101. The C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years).

Application

The Engineered Control cells can be used for drug permeability assays. They may also be used as the control in conjunction with C2BBe1 Transporter Knockout cell lines for drug/transporter interaction studies. Engineered control cells have been used in the in vitro Caco-2 permeability assay. The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases

Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes

Caco-2 Transporter Knockout Cell Based Assays

Features and Benefits

The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human-derived, and grow in a homogenous monolayer that forms tight junctions which is necessary for efflux ratio analysis.

Quality

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

Legal Information

This product is covered under a consumable purchase agreement for one-time use only. For more information:ADME/Tox Cell Lines License

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable